Small buccal fat pad cells have high osteogenic differentiation potential

Niina Tsurumachi, Daisuke Akita, Koichiro Kano, Taro Matsumoto, Taku Toriumi, Tomohiko Kazama, Yoshinao Oki, Yoko Tamura, Morio Tonogi, Keitaro Isokawa, Noriyoshi Shimizu, Masaki Honda

研究成果: ジャーナルへの寄稿記事査読

17 被引用数 (Scopus)


Dedifferentiated fat (DFAT) cells derived from mature adipocytes have mesenchymal stem cells' (MSCs) characteristics. Generally, mature adipocytes are 60-110 μm in diameter; however, association between adipocyte size and dedifferentiation efficiency is still unknown. This study, therefore, investigated the dedifferentiation efficiency of adipocytes based on cell diameter. Buccal fat pad was harvested from five human donors and dissociated by collagenase digestion. After exclusion of unwanted stromal cells by centrifugation, floating adipocytes were collected and their size distribution was analyzed. The floating adipocytes were then separated into two groups depending on cell size using 40- and 100-μm nylon mesh filters: cell diameters less than 40 μm (small adipocytes: S-adipocytes) and cell diameters of 40-100 μm (large adipocytes: L-adipocytes). Finally, we evaluated the efficiency of adipocyte dedifferentiation and then characterized the resultant DFAT cells. The S-adipocytes showed a higher capacity to dedifferentiate into DFAT cells (S-DFAT cells) compared to the L-adipocytes (L-DFAT cells). The S-DFAT cells also showed a relatively higher proportion of CD146-positive cells than L-DFAT cells, and exhibited more osteogenic differentiation ability based on the alkaline phosphatase activity and amount of calcium deposition. These results suggested that the S- and L-DFAT cells had distinct characteristics, and that the higher dedifferentiation potential of S-adipocytes compared to L-adipocytes gives the former group an advantage in yielding DFAT cells.

ジャーナルTissue Engineering - Part C: Methods
出版ステータス出版済み - 3月 2016


「Small buccal fat pad cells have high osteogenic differentiation potential」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。