TY - GEN
T1 - Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells
AU - Hsiao, Amy Y.
AU - Okitsu, Teru
AU - Onoe, Hiroaki
AU - Kiyosawa, Mahiro
AU - Teramae, Hiroki
AU - Iwanaga, Shintaroh
AU - Miura, Shigenori
AU - Kazama, Tomohiko
AU - Matsumoto, Taro
AU - Takeuchi, Shoji
PY - 2013
Y1 - 2013
N2 - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.
AB - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.
UR - http://www.scopus.com/inward/record.url?scp=84875435888&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2013.6474179
DO - 10.1109/MEMSYS.2013.6474179
M3 - Conference contribution
AN - SCOPUS:84875435888
SN - 9781467356558
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 71
EP - 73
BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Y2 - 20 January 2013 through 24 January 2013
ER -