No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

Meripet Polat Yamanaka, Susumu Saito, Yukiko Hara, Ryosuke Matsuura, Shin nosuke Takeshima, Kazuyoshi Hosomichi, Yasunobu Matsumoto, Rika A. Furuta, Masami Takei, Yoko Aida

研究成果: ジャーナルへの寄稿記事査読

5 被引用数 (Scopus)

抄録

Background: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. Result: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Conclusion: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.

本文言語英語
論文番号7
ジャーナルRetrovirology
19
1
DOI
出版ステータス出版済み - 12月 2022

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