Multiple cleavage sites for polymeric immunoglobulin receptor

Masatake Asano, Nobuko Takenouchi-Ohkubo, Naoyuki Matsumoto, Yoshitaka Ogura, Hirofumi Nomura, Hisashi Suguro, Itaru Moro

研究成果: ジャーナルへの寄稿記事査読

11 被引用数 (Scopus)

抄録

Human polymeric immunoglobulin receptor (pIgR) was expressed in baby hamster kidney (BHK) cells using a recombinant vaccinia virus transfection system. Cleavage of pIgR on the cell surface was partially inhibited by the proteinase inhibitor, leupeptin. We addressed the question whether some particular regions of pIgR could affect the efficient cleavage of this molecule, with the following results: (1) a mutant lacking the entire cytoplasmic region resulted in release of secretory component (SC) into the culture supernatant much faster than wild-type; (2) a pIgR mutant lacking the entire extracellular domain 6, the region containing the susceptible cleavage sites, could be cleaved and released as a mutant SC. The transport kinetics of this mutant between endoplasmic reticulum (ER) and Golgi or Golgi and the cell surface was equivalent to wild-type pIgR. Our results indicate that although the main cleavage site is in domain 6, at least one other cleavage site may exist.

本文言語英語
ページ(範囲)583-589
ページ数7
ジャーナルImmunology
112
4
DOI
出版ステータス出版済み - 8月 2004

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