Molecular cloning of the canine telomerase reverse transcriptase gene and its expression in neoplastic and non-neoplastic cells

Mitsuhiro Yazawa, Masaru Okuda, Noriko Kanaya, Sung Hyeok Hong, Tomoko Takahashi, Emi Ohashi, Takayuki Nakagawa, Ryohei Nishimura, Nobuo Sasaki, Kenichi Masuda, Koichi Ohno, Hajime Tsujimoto

研究成果: ジャーナルへの寄稿記事査読

18 被引用数 (Scopus)

抄録

Objective - To perform molecular cloning of the canine telomerase reverse transcriptase (TERT) gene and determine its expression in neoplastic and non-neoplastic cells. Sample Population - 9 canine tumor cell lines derived from various neoplasms, 16 primary canine tumors, and tissues from 15 normal canine organs. Procedure - Tumor cell lines were derived from canine tumors that included osteosarcoma, mammary gland adenocarcinoma, melanoma, acute lymphoblastic leukemia, lymphoma, and mastocytoma and a canine primary fibroblast culture. Canine TERT complementary DNA (cDNA) was amplified by use of polymerase chain reaction (PCR) and sequenced. Expression of TERT mRNA was examined by reverse transcription (RT)-PCR assay. Telomerase activity was measured by use of the telomeric repeat amplification protocol assay. Results - The canine TERT cDNA clone was 237 base pairs in length and contained a central region encoding the reverse transcriptase motif 2. Expression of TERT mRNA was detected in canine tumor cell lines that had telomerase activity but not in telomerase-negative canine primary fibroblasts. The TERT mRNA was detected in 13 of 16 canine tumor tissues and several normal tissues such as liver, ovary, lymph node, and thymus. A significant correlation between TERT expression level and telomerase activity was noted. Conclusions and Clinical Relevance - Expression of TERT mRNA was closely associated with telomerase activity in neoplastic cells as well as some non-neoplastic cells from dogs. In addition to telomerase activity, expression of TERT mRNA can be used as a marker of tumor cells.

本文言語英語
ページ(範囲)1395-1400
ページ数6
ジャーナルAmerican Journal of Veterinary Research
64
11
DOI
出版ステータス出版済み - 11月 2003
外部発表はい

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