MicroRNA-125b regulates proliferation and radioresistance of oral squamous cell carcinoma

M. Shiiba, K. Shinozuka, K. Saito, K. Fushimi, A. Kasamatsu, K. Ogawara, K. Uzawa, H. Ito, Y. Takiguchi, H. Tanzawa

研究成果: ジャーナルへの寄稿記事査読

104 被引用数 (Scopus)

抄録

Background: MicroRNAs (miRNAs) are involved in essential biological activities, and have been reported to exhibit differential expression profiles in various cancers. Our previous study demonstrated that intercellular adhesion molecule-2 (ICAM2) inhibition induces radiosensitisation in oral squamous cell carcinoma (OSCC) cells. Thus, we hypothesised that certain miRNAs play crucial roles in radioresistance in OSCC by regulating ICAM2 expression. Methods: Because predicted target gene analyses revealed that microRNA-125b (miR-125b) potentially regulates ICAM2 mRNA expression, we examined the association between miR-125b and radioresistance. The expression of miR-125b was investigated by real-time quantitative reverse transcriptase-PCR. For a functional analysis, miR-125b was transfected to OSCC-derived cells. Results: A downregulated expression of miR-125b was found in OSCC-derived cell lines and OSCC samples. The miR-125b-transfected cells showed a decreased proliferation rate, enhanced radiosensitivity to X-ray irradiation and diminished ICAM2 mRNA expression. Moreover, miR-125b expression correlated with OSCC tumour staging and survival. Conclusion: These findings suggested that the downregulated miR-125b expression was associated with proliferation and radioresistance mechanisms, probably through ICAM2 signalling. Thus, controlling the expression or activity of miR-125b might contribute to suppressing proliferation and overcoming radioresistance in OSCC.

本文言語英語
ページ(範囲)1817-1821
ページ数5
ジャーナルBritish Journal of Cancer
108
9
DOI
出版ステータス出版済み - 14 5月 2013
外部発表はい

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