Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro

Tomohiko Kazama, Masaki Fujie, Tuyoshi Endo, Koichiro Kano

研究成果: ジャーナルへの寄稿記事査読

87 被引用数 (Scopus)

抄録

We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro.

本文言語英語
ページ(範囲)780-785
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
377
3
DOI
出版ステータス出版済み - 19 12月 2008

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