Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

Soichiro Manaka; Natsuko Tanabe; Taro Kariya; Masako Naito; Tadahiro Takayama; Mayu Nagao; Di Liu; Koichi Ito; Masao Maeno; Naoto Suzuki; Masashi Miyazaki

doi:10.1016/j.febslet.2014.12.013

Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

Soichiro Manaka, Natsuko Tanabe, Taro Kariya, Masako Naito, Tadahiro Takayama, Mayu Nagao, Di Liu, Koichi Ito, Masao Maeno, Naoto Suzuki, Masashi Miyazaki

歯学部

研究成果: ジャーナルへの寄稿 › 記事 › 査読

46 被引用数 (Scopus)

抄録

Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

本文言語	英語
ページ（範囲）	310-318
ページ数	9
ジャーナル	FEBS Letters
巻	589
号	3
DOI	https://doi.org/10.1016/j.febslet.2014.12.013
出版ステータス	出版済み - 30 1月 2015

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10.1016/j.febslet.2014.12.013

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引用スタイル

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title = "Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells",

abstract = "Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.",

keywords = "Adenosine triphosphate, Low-intensity pulsed ultrasound, Osteogenesis, P2X7",

author = "Soichiro Manaka and Natsuko Tanabe and Taro Kariya and Masako Naito and Tadahiro Takayama and Mayu Nagao and Di Liu and Koichi Ito and Masao Maeno and Naoto Suzuki and Masashi Miyazaki",

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T1 - Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

AU - Manaka, Soichiro

AU - Tanabe, Natsuko

AU - Kariya, Taro

AU - Naito, Masako

AU - Takayama, Tadahiro

AU - Nagao, Mayu

AU - Liu, Di

AU - Ito, Koichi

AU - Maeno, Masao

AU - Suzuki, Naoto

AU - Miyazaki, Masashi

PY - 2015/1/30

Y1 - 2015/1/30

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AB - Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

KW - Adenosine triphosphate

KW - Low-intensity pulsed ultrasound

KW - Osteogenesis

KW - P2X7

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