TY - JOUR
T1 - Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella
AU - Hashizume, Tomomi
AU - Momoi, Fumiki
AU - Kurita-Ochiai, Tomoko
AU - Kaminogawa, Shuichi
AU - Hosono, Akira
AU - Kataoka, Kosuke
AU - Shinozaki-Kuwahara, Noriko
AU - Kweon, Mi Na
AU - Yamamoto, Masafumi
PY - 2007/8/24
Y1 - 2007/8/24
N2 - In this study, we investigated whether isolated lymphoid follicles (ILF) play a role in the regulation of intestinal IgA antibody (Ab) responses. The transfer of wild type (WT) bone marrow (BM) to lymphotoxin-α-deficient (LTα-/-) mice resulted in the formation of mature ILF containing T cells, B cells, and FDC clusters in the absence of mesenteric lymph nodes and Peyer's patches. Although the ILF restored total IgA Abs in the intestine, antigen (Ag)-specific IgA responses were not induced after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin. Moreover, Ag-specific cell proliferation was not detected in the ILF. Interestingly, no IgA anti-LPS Abs were detected in the fecal extracts of LTα-/- mice reconstituted with WT BM. On the basis of these findings, ILF can be presumed to play a role in the production of IgA Abs, but lymphoid nodules are not inductive sites for the regulation of Ag-specific intestinal IgA responses to recombinant Salmonella.
AB - In this study, we investigated whether isolated lymphoid follicles (ILF) play a role in the regulation of intestinal IgA antibody (Ab) responses. The transfer of wild type (WT) bone marrow (BM) to lymphotoxin-α-deficient (LTα-/-) mice resulted in the formation of mature ILF containing T cells, B cells, and FDC clusters in the absence of mesenteric lymph nodes and Peyer's patches. Although the ILF restored total IgA Abs in the intestine, antigen (Ag)-specific IgA responses were not induced after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin. Moreover, Ag-specific cell proliferation was not detected in the ILF. Interestingly, no IgA anti-LPS Abs were detected in the fecal extracts of LTα-/- mice reconstituted with WT BM. On the basis of these findings, ILF can be presumed to play a role in the production of IgA Abs, but lymphoid nodules are not inductive sites for the regulation of Ag-specific intestinal IgA responses to recombinant Salmonella.
KW - IgA
KW - Isolated lymphoid follicles
KW - Recombinant Salmonella
UR - http://www.scopus.com/inward/record.url?scp=34447103299&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.06.096
DO - 10.1016/j.bbrc.2007.06.096
M3 - Article
C2 - 17606222
AN - SCOPUS:34447103299
SN - 0006-291X
VL - 360
SP - 388
EP - 393
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -