TY - JOUR
T1 - Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid
AU - Takenouchi, Y.
AU - Ohshima, M.
AU - Yamaguchi, Y.
AU - Nishida, T.
AU - Senda, N.
AU - Idesawa, M.
AU - Otsuka, K.
AU - Ito, K.
PY - 2010/12
Y1 - 2010/12
N2 - Objective: Insulin-like growth factor-binding proteins (IGFBPs) are crucial regulators of insulin-like growth factor (IGF). They enhance or inhibit IGF functions, but also exhibit IGF-independent effects. In a previous study, we detected, qualitatively, IGFBP-2 and -3 in gingival crevicular fluid using a cytokine antibody array. Here we extended these results using an ELISA to determine the concentrations of IGFBP-2 and -3 in gingival crevicular fluid. In addition, we explored whether the expression of IGFBP-2 and IGFBP-3 correlates with periodontal disease severity.Material and Methods: Gingival crevicular fluid samples from 92 sites of 12 patients affected with periodontal disease and from 100 sites of 19 healthy volunteers, were collected, divided into two groups and analyzed by ELISA for IGFBP-2 and -3 expression. The potential correlation among probing depth, gingival index and the concentrations of IGFBP-2 and -3 was analyzed.Results: Positive correlations were observed between the concentration of IGFBP-2 and probing depth and gingival index, but not for IGFBP-3. The IGFBP-2 concentrations at bleeding on probing-positive sites and at sites with a probing depth of ≥4mm were higher than at bleeding on probing-negative sites and at sites with a probing depth of ≤3mm.Conclusion: These results indicate that IGFBP-2 is a potential novel marker for periodontal disease progression. As IGFBP-2 modulates bone metabolism and cell migration, IGFBP-2 in the gingival crevicular fluid may reflect periodontal disease activity.
AB - Objective: Insulin-like growth factor-binding proteins (IGFBPs) are crucial regulators of insulin-like growth factor (IGF). They enhance or inhibit IGF functions, but also exhibit IGF-independent effects. In a previous study, we detected, qualitatively, IGFBP-2 and -3 in gingival crevicular fluid using a cytokine antibody array. Here we extended these results using an ELISA to determine the concentrations of IGFBP-2 and -3 in gingival crevicular fluid. In addition, we explored whether the expression of IGFBP-2 and IGFBP-3 correlates with periodontal disease severity.Material and Methods: Gingival crevicular fluid samples from 92 sites of 12 patients affected with periodontal disease and from 100 sites of 19 healthy volunteers, were collected, divided into two groups and analyzed by ELISA for IGFBP-2 and -3 expression. The potential correlation among probing depth, gingival index and the concentrations of IGFBP-2 and -3 was analyzed.Results: Positive correlations were observed between the concentration of IGFBP-2 and probing depth and gingival index, but not for IGFBP-3. The IGFBP-2 concentrations at bleeding on probing-positive sites and at sites with a probing depth of ≥4mm were higher than at bleeding on probing-negative sites and at sites with a probing depth of ≤3mm.Conclusion: These results indicate that IGFBP-2 is a potential novel marker for periodontal disease progression. As IGFBP-2 modulates bone metabolism and cell migration, IGFBP-2 in the gingival crevicular fluid may reflect periodontal disease activity.
KW - Gingival crevicular fluid
KW - Insulin-like growth factor-binding protein-2
KW - Insulin-like growth factor-binding protein-3
KW - Marker
KW - Periodontal disease
UR - http://www.scopus.com/inward/record.url?scp=77958531659&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0765.2010.01309.x
DO - 10.1111/j.1600-0765.2010.01309.x
M3 - Article
C2 - 20860589
AN - SCOPUS:77958531659
SN - 0022-3484
VL - 45
SP - 803
EP - 808
JO - Journal of Periodontal Research
JF - Journal of Periodontal Research
IS - 6
ER -