TY - JOUR
T1 - Inhibition of Epithelial-Mesenchymal Transition Maintains Stemness in Human Amniotic Epithelial Cells
AU - Takano, Chika
AU - Horie, Masafumi
AU - Taiko, Isamu
AU - Trinh, Quang Duy
AU - Kanemaru, Kazunori
AU - Komine-Aizawa, Shihoko
AU - Hayakawa, Satoshi
AU - Miki, Toshio
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Human amniotic epithelial cells (hAECs), which are a type of placental stem cell, express stem cell marker genes and are capable of differentiating into all three germ layers under appropriate culture conditions. hAECs are known to undergo TGF-β-dependent epithelial-mesenchymal transition (EMT); however, the impact of EMT on the stemness or differentiation of hAECs has not yet been determined. Here, we first confirmed that hAECs undergo EMT immediately after starting primary culture. Comprehensive transcriptome analysis using RNA-seq revealed that inhibition of TGF-β-dependent EMT maintained the expression of stemness-related genes, including NANOG and POU5F1, in hAECs. Moreover, the maintenance of stemness did not affect the nontumorigenic characteristics of hAECs. We showed for the first time that TGF-β-dependent EMT negatively affected the stemness of hAECs, providing novel insight into cellular processes of placental stem cells. Graphical abstract: [Figure not available: see fulltext.]
AB - Human amniotic epithelial cells (hAECs), which are a type of placental stem cell, express stem cell marker genes and are capable of differentiating into all three germ layers under appropriate culture conditions. hAECs are known to undergo TGF-β-dependent epithelial-mesenchymal transition (EMT); however, the impact of EMT on the stemness or differentiation of hAECs has not yet been determined. Here, we first confirmed that hAECs undergo EMT immediately after starting primary culture. Comprehensive transcriptome analysis using RNA-seq revealed that inhibition of TGF-β-dependent EMT maintained the expression of stemness-related genes, including NANOG and POU5F1, in hAECs. Moreover, the maintenance of stemness did not affect the nontumorigenic characteristics of hAECs. We showed for the first time that TGF-β-dependent EMT negatively affected the stemness of hAECs, providing novel insight into cellular processes of placental stem cells. Graphical abstract: [Figure not available: see fulltext.]
KW - Comprehensive transcriptome analysis
KW - Epithelial-mesenchymal transition
KW - Human amniotic epithelial cells
KW - Stemness
KW - TGF-β pathway
UR - http://www.scopus.com/inward/record.url?scp=85135476315&partnerID=8YFLogxK
U2 - 10.1007/s12015-022-10420-1
DO - 10.1007/s12015-022-10420-1
M3 - Article
C2 - 35931939
AN - SCOPUS:85135476315
SN - 2629-3269
VL - 18
SP - 3083
EP - 3091
JO - Stem Cell Reviews and Reports
JF - Stem Cell Reviews and Reports
IS - 8
ER -
