TY - JOUR
T1 - Genetic diversity of Parechovirus A in infants and children with acute gastroenteritis in Japan during 2016–2018
AU - Pham, Ngan Thi Kim
AU - Thongprachum, Aksara
AU - Shimizu, Yuko
AU - Shiota, Itoe
AU - Hoque, Sheikh Ariful
AU - Khamrin, Pattara
AU - Takano, Chika
AU - Trinh, Quang Duy
AU - Okitsu, Shoko
AU - Komine-Aizawa, Shihoko
AU - Shimizu, Hiroyuki
AU - Maneekarn, Niwat
AU - Hayakawa, Satoshi
AU - Ushijima, Hiroshi
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/6
Y1 - 2021/6
N2 - Parechovirus A (PeV-A), previously known as human parechovirus, is a common pathogen in children that can cause respiratory and gastrointestinal diseases as well as severe neurological disease. Take advantage of our previous findings on the genetic diversity of PeV-A circulating in Japanese children with acute gastroenteritis (AGE), this study was conducted to investigate the genetic diversity of PeV-A isolated from children with AGE in Japan as well as their clinical symptoms. Of 1070 stool samples collected from Japanese infants and children with AGE during the 2-year period from July 2016 to June 2018, 76 were positive for PeV-A by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) and were subjected to genotyping based on viral protein 1 (VP1) sequences. Five different PeV-A genotypes including PeV-A1B, −A2, −A3, −A4, and -A6 were detected with predominant of PeV-A1 clade B genotype. This study revealed a high genetic diversity of PeV-A circulating in Japanese infants and children with AGE and the PeV-A2, a rare genotype, was detected for the first time in Japan in patients with AGE. The clinical symptoms observed in these patients included diarrhea, vomiting, fever, cough, rhinorrhea, and dehydration.
AB - Parechovirus A (PeV-A), previously known as human parechovirus, is a common pathogen in children that can cause respiratory and gastrointestinal diseases as well as severe neurological disease. Take advantage of our previous findings on the genetic diversity of PeV-A circulating in Japanese children with acute gastroenteritis (AGE), this study was conducted to investigate the genetic diversity of PeV-A isolated from children with AGE in Japan as well as their clinical symptoms. Of 1070 stool samples collected from Japanese infants and children with AGE during the 2-year period from July 2016 to June 2018, 76 were positive for PeV-A by multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) and were subjected to genotyping based on viral protein 1 (VP1) sequences. Five different PeV-A genotypes including PeV-A1B, −A2, −A3, −A4, and -A6 were detected with predominant of PeV-A1 clade B genotype. This study revealed a high genetic diversity of PeV-A circulating in Japanese infants and children with AGE and the PeV-A2, a rare genotype, was detected for the first time in Japan in patients with AGE. The clinical symptoms observed in these patients included diarrhea, vomiting, fever, cough, rhinorrhea, and dehydration.
KW - Acute gastroenteritis
KW - Children
KW - Genotype
KW - Japan
KW - PeV-A
UR - http://www.scopus.com/inward/record.url?scp=85101330383&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2021.104776
DO - 10.1016/j.meegid.2021.104776
M3 - Article
C2 - 33621710
AN - SCOPUS:85101330383
SN - 1567-1348
VL - 90
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 104776
ER -
