TY - GEN
T1 - Fluctuation of actin sliding over myosin thick filaments in vitro
AU - Noda, Naoki
AU - Imafuku, Yasuhiro
AU - Yamada, Akira
AU - Tawada, Katsuhisa
PY - 2006
Y1 - 2006
N2 - It is customarily assumed that myosin motors act as independent force generators. Here, we tested this assumption by analyzing the fluctuation of the actin sliding movement in vitro over long native thick filaments from shell fish smooth muscle. This analysis is based on the prediction that the effective diffusion coefficient of actin, a measure of the fluctuation, is proportional to the inverse of the number of myosin motors generating the sliding movement of an actin filament, hence proportional to the inverse of the actin length, if the actions of the motors are independent. Contrary to this prediction, we found the effective diffusion coefficient to be independent of the actin length. This result shows that the myosin motors are not independent force generators when generating the continuous sliding movement of actin in vitro.
AB - It is customarily assumed that myosin motors act as independent force generators. Here, we tested this assumption by analyzing the fluctuation of the actin sliding movement in vitro over long native thick filaments from shell fish smooth muscle. This analysis is based on the prediction that the effective diffusion coefficient of actin, a measure of the fluctuation, is proportional to the inverse of the number of myosin motors generating the sliding movement of an actin filament, hence proportional to the inverse of the actin length, if the actions of the motors are independent. Contrary to this prediction, we found the effective diffusion coefficient to be independent of the actin length. This result shows that the myosin motors are not independent force generators when generating the continuous sliding movement of actin in vitro.
UR - http://www.scopus.com/inward/record.url?scp=50449084633&partnerID=8YFLogxK
U2 - 10.1109/MHS.2006.320292
DO - 10.1109/MHS.2006.320292
M3 - Conference contribution
AN - SCOPUS:50449084633
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -