Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines

Munekazu Nakaichi, Yoko Takeshita, Masaru Okuda, Yuya Nakamoto, Kazuhito Itamoto, U. N.E. Satoshi, Nobuo Sasaki, Tsuyoshi Kadosawa, Tomoko Takahashi, Yasuho Taura

研究成果: ジャーナルへの寄稿記事査読

18 被引用数 (Scopus)

抄録

Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors.

本文言語英語
ページ(範囲)111-115
ページ数5
ジャーナルJournal of Veterinary Medical Science
69
2
DOI
出版ステータス出版済み - 2月 2007

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