Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells

Ayano Hidaka, Shota Uekusa, Takashi Hosokawa, Hide Kaneda, Tomohiko Kazama, Kazuhiro Hagikura, Shuichiro Uehara, Tsugumichi Koshinaga, Taro Matsumoto

研究成果: ジャーナルへの寄稿記事査読

1 被引用数 (Scopus)

抄録

Purpose: Mesenchymal stem cells (MSCs) can induce differentiation of neuroblastoma (NB) cells. Properties of dedifferentiated fat cells (DFATs) are similar to those of MSCs. Here, we investigated whether DFATs can induce NB cell differentiation and suppress cell proliferation. Methods: DFATs were obtained from mature adipocytes isolated from adipose tissue from a ceiling culture. NB cells were cultured in a medium with or without DFATs and, subsequently, cultured in a DFAT-conditioned medium (CM) with or without phosphatidylinositol 3-kinase (PI3K) inhibitor. The neurite lengths were measured, and mRNA expression levels of the neurofilament (NF) and tubulin beta III (TUBβ3) were assessed using quantitative real-time RT-PCR. Cell viability was assessed using the WST-1 assay. Results: NB cells cultured with DFATs caused elongation of the neurites and upregulated the expression of NF and Tubβ3. NB cells cultured in DFAT-CM demonstrated increased cell viability. NB cells cultured with DFAT-CM and PI3K inhibitors suppressed cell viability. NB cells cultured with DFAT-CM and PI3K inhibitor demonstrated increased neurite length and expression, and upregulation of Tubβ3. Conclusion: The combined use of DFAT-CM and PI3K inhibitors suppresses the proliferation of NB cells and induces their differentiation. Thus, DFAT may offer new insights into therapeutic approaches in neuroblastoma.

本文言語英語
論文番号58
ジャーナルPediatric Surgery International
39
1
DOI
出版ステータス出版済み - 12月 2023

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