DNA repair and STR PCR amplification from damaged DNA of human bloodstains

Jian Tie, Seisaku Uchigasaki

研究成果: ジャーナルへの寄稿記事査読

6 被引用数 (Scopus)

抄録

Detection and identification of DNA structure from aged and damaged biological materials such as bloodstain are important for human genetic study and individual identification. However, after a long period of storage, the DNA structure of biological samples is degraded to various degrees depending on several factors including environmental condition. In this study, human bloodstains that have been stored at room temperature for one to 39 years were used to represent damaged biological samples. The numbers of apurinic/apyrimidinic sites (AP sites) were investigated by the DNA Damage Quantification Kit to evaluate the lesions in DNA structure. The damaged DNA from the stored human bloodstains was repaired using seven DNA repair enzymes. As DNA genetic marker, short tandem repeat (STR) genotypes were amplified using the non-repaired and repaired DNA preparations from the stored bloodstains. The results indicated that the number of AP sites increased as the storage time increased. While only 2 to 6 STR loci were detected in the damaged DNA of bloodstains stored for over 30 years, after DNA repair all the genotypes in the STR system could be analyzed even from bloodstains that had been stored for the longest period.

本文言語英語
ページ(範囲)1505-1510
ページ数6
ジャーナルMolecular Biology Reports
40
2
DOI
出版ステータス出版済み - 2月 2013

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