TY - JOUR
T1 - Corrigendum to “Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration” [Regen Ther 11 (2019) 240–248] (Regenerative Therapy (2019) 11 (240–248), (S2352320419300781), (10.1016/j.reth.2019.08.004))
AU - Fujimaki, Hiroshi
AU - Matsumine, Hajime
AU - Osaki, Hironobu
AU - Ueta, Yoshifumi
AU - Kamei, Wataru
AU - Shimizu, Mari
AU - Hashimoto, Kazuki
AU - Fujii, Kaori
AU - Kazama, Tomohiko
AU - Matsumoto, Taro
AU - Niimi, Yosuke
AU - Miyata, Mariko
AU - Sakurai, Hiroyuki
N1 - Publisher Copyright:
© 2020 The Japanese Society for Regenerative Medicine
PY - 2020/12
Y1 - 2020/12
N2 - Introduction: Polyglycolic acid (PGA) nerve conduits, an artificial biodegradable nerve regeneration-inducing tube currently used in clinical practice, are effective in regenerating peripheral nerves. Dedifferentiated fat (DFAT) cells differentiate into various cells including adipocytes, osteoblasts, chondrocytes, skeletal muscle cells, and myofibroblasts, when cultured in appropriate differentiation-inducing conditioned culture medium. This study made a hybrid artificial nerve conduit by filling a PGA conduit with DFAT cells, applied the conduit to a rat facial nerve defect model, and investigated the facial nerve regenerative ability of the conduit. Methods: Under inhalational anesthesia, the buccal branch of the facial nerve in Lewis rats was exposed, and a 7-mm nerve defect was created. PGA nerve conduits were filled with DFAT cells, which were prepared from rat subcutaneous adipose tissue with type I collagen as a scaffold, and then grafted into the nerve defect sites in rats with a microscope (DFAT group) (n = 10). In other rats, PGA artificial nerve conduits alone were similarly grafted into the nerve defect sites (the control group) (n = 10). Reinnervation was confirmed at 13 weeks postoperatively by a retrograde tracer, followed by histological and physiological comparative studies. Results: The mean number of myelinated fibers was significantly higher in DFAT group (1606 ± 806) than in the control group (543 ± 478). Myelin thickness was also significantly lager in DFAT group (0.67 ± 0.02 μm) than in the control group (0.43 ± 0.01 μm). Although no significant difference was found in the amplitude of compound muscle action potential (CMAP) between DFAT group (2.84 ± 2.47 mV) and the control group (0.88 ± 0.56 mV), whisker motion was lager in DFAT group (9.22° ± 0.65°) than in the control group (1.9° ± 0.84°). Conclusions: DFAT cell-filled PGA conduits were found to promote nerve regeneration in an experimental rat facial nerve defect model.
AB - Introduction: Polyglycolic acid (PGA) nerve conduits, an artificial biodegradable nerve regeneration-inducing tube currently used in clinical practice, are effective in regenerating peripheral nerves. Dedifferentiated fat (DFAT) cells differentiate into various cells including adipocytes, osteoblasts, chondrocytes, skeletal muscle cells, and myofibroblasts, when cultured in appropriate differentiation-inducing conditioned culture medium. This study made a hybrid artificial nerve conduit by filling a PGA conduit with DFAT cells, applied the conduit to a rat facial nerve defect model, and investigated the facial nerve regenerative ability of the conduit. Methods: Under inhalational anesthesia, the buccal branch of the facial nerve in Lewis rats was exposed, and a 7-mm nerve defect was created. PGA nerve conduits were filled with DFAT cells, which were prepared from rat subcutaneous adipose tissue with type I collagen as a scaffold, and then grafted into the nerve defect sites in rats with a microscope (DFAT group) (n = 10). In other rats, PGA artificial nerve conduits alone were similarly grafted into the nerve defect sites (the control group) (n = 10). Reinnervation was confirmed at 13 weeks postoperatively by a retrograde tracer, followed by histological and physiological comparative studies. Results: The mean number of myelinated fibers was significantly higher in DFAT group (1606 ± 806) than in the control group (543 ± 478). Myelin thickness was also significantly lager in DFAT group (0.67 ± 0.02 μm) than in the control group (0.43 ± 0.01 μm). Although no significant difference was found in the amplitude of compound muscle action potential (CMAP) between DFAT group (2.84 ± 2.47 mV) and the control group (0.88 ± 0.56 mV), whisker motion was lager in DFAT group (9.22° ± 0.65°) than in the control group (1.9° ± 0.84°). Conclusions: DFAT cell-filled PGA conduits were found to promote nerve regeneration in an experimental rat facial nerve defect model.
KW - Adipose tissue
KW - DFAT cells
KW - Nerve regeneration
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=85086146952&partnerID=8YFLogxK
U2 - 10.1016/j.reth.2020.05.002
DO - 10.1016/j.reth.2020.05.002
M3 - Comment/debate
AN - SCOPUS:85086146952
SN - 2352-3204
VL - 15
SP - 35
EP - 43
JO - Regenerative Therapy
JF - Regenerative Therapy
ER -