A new microscope optics for laser dark-field illumination applied to high precision two dimensional measurement of specimen displacement

Naoki Noda, Shinji Kamimura

研究成果: ジャーナルへの寄稿記事査読

12 被引用数 (Scopus)

抄録

With conventional light microscopy, precision in the measurement of the displacement of a specimen depends on the signal-to-noise ratio when we measure the light intensity of magnified images. This implies that, for the improvement of precision, getting brighter images and reducing background light noise are both inevitably required. For this purpose, we developed a new optics for laser dark-field illumination. For the microscopy, we used a laser beam and a pair of axicons (conical lenses) to get an optimal condition for dark-field observations. The optics was applied to measuring two dimensional microbead displacements with subnanometer precision. The bandwidth of our detection system overall was 10 kHz. Over most of this bandwidth, the observed noise level was as small as 0.1 nmHz.

本文言語英語
論文番号023704
ジャーナルReview of Scientific Instruments
79
2
DOI
出版ステータス出版済み - 2008
外部発表はい

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