Ultrastructural immunolocalization of laminin 332 (laminin 5) at dento-gingival interface in Macaca fuscata monkey

Takashi Sawada, Takaki Yamazaki, Kazuko Shibayama, Yoko Yamaguchi, Mitsuhiro Ohshima

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Although laminin 332 (laminin 5), an extracellular matrix molecule involved in cell adhesion and migration, has been localized at the interface between the tooth enamel and junctional epithelium, its ultrastructural localization remains to be fully clarified. The purpose of the present study was to investigate the ultrastructural distribution of laminin 332 at the dento-gingival interface in Japanese monkey (Macaca fuscata) using pre- and post-embedding immunoelectron microscopy. Pre-embedding immunoelectron microscopy revealed a broad band of internal basal lamina together with supplementary lamina densa, and both showed immunolabeling for laminin 332. Immunoreaction products for laminin 332 were observed in the rough-surfaced endoplasmic reticulum of the junctional epithelial cells close to the tooth enamel. Post-embedding immunoelectron microscopy revealed an increase in the number of immunogold particles toward the coronal portion, resulting in a large accumulation of particles on the basal lamina, preferentially on the lamina densa. Concomitantly the dental cuticle at the dento-gingival interface was sporadically, but specifically, immunogold-labeled with anti-laminin 332 antibody. These data suggest that junctional epithelium actively produces laminin 332, and that the products accumulate at the dento-gingival interface during cell migration coronally towards the gingival sulcus.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalMedical Molecular Morphology
Issue number2
Publication statusPublished - 1 Jun 2015


  • Dental cuticle
  • Immunoelectron microscopy
  • Junctional epithelium
  • Laminin 332
  • Macaca fuscata
  • Primate


Dive into the research topics of 'Ultrastructural immunolocalization of laminin 332 (laminin 5) at dento-gingival interface in Macaca fuscata monkey'. Together they form a unique fingerprint.

Cite this