TRPA1 contributes to capsaicin-induced facial cold hyperalgesia in rats

Kuniya Honda, Masamichi Shinoda, Akihiko Furukawa, Kozue Kita, Noboru Noma, Koichi Iwata

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Orofacial cold hyperalgesia is known to cause severe persistent pain in the face following trigeminal nerve injury or inflammation, and transient receptor potential (TRP) vanilloid 1 (TRPV1) and TRP ankylin 1 (TRPA1) are thought to be involved in cold hyperalgesia. However, how these two receptors are involved in cold hyperalgesia is not fully understood. To clarify the mechanisms underlying facial cold hyperalgesia, nocifensive behaviors to cold stimulation, the expression of TRPV1 and TRPA1 in trigeminal ganglion (TG) neurons, and TG neuronal excitability to cold stimulation following facial capsaicin injection were examined in rats. The head-withdrawal reflex threshold (HWRT) to cold stimulation of the lateral facial skin was significantly decreased following facial capsaicin injection. This reduction of HWRT was significantly recovered following local injection of TRPV1 antagonist as well as TRPA1 antagonist. Approximately 30% of TG neurons innervating the lateral facial skin expressed both TRPV1 and TRPA1, and about 64% of TRPA1-positive neurons also expressed TRPV1. The TG neuronal excitability to noxious cold stimulation was significantly increased following facial capsaicin injection and this increase was recovered by pretreatment with TRPA1 antagonist. These findings suggest that TRPA1 sensitization via TRPV1 signaling in TG neurons is involved in cold hyperalgesia following facial skin capsaicin injection.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalEuropean Journal of Oral Sciences
Issue number6
Publication statusPublished - 1 Dec 2014


  • Orofacial pain
  • Transient receptor potential ankylin 1
  • Transient receptor potential vanilloid 1
  • Trigeminal ganglion


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