Synthetic pyrrole-imidazole polyamide inhibits expression of the human transforming growth factor-β1 gene

Yu Mu Lai, Noboru Fukuda, Takahiro Ueno, Hiroyuki Matsuda, Satoshi Saito, Koichi Matsumoto, Hirohito Ayame, Toshikazu Bando, Hiroshi Sugiyama, Hideo Mugishima, Kazuo Serie

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Pyrrole-imidazole (Py-Im) polyamides can bind to the predetermined base pairs in the minor groove of double-helical DNA with high affinity. These synthetic small molecules can interfere with transcription factor-DNA interaction and inhibit or activate the transcription of corresponding genes. In the present study, we designed and synthesized a Py-Im polyamide to target -545 to -539 base pairs of human transforming growth factor-β1 (hTGF-β1) promoter adjacent to the fat-specific element 2 (FSE2) to inhibit the expression of the gene. Gel mobility shift assay showed that the synthetic Py-Im polyamide binds to its corresponding double-strand oligonucleotides, whereas the mismatch polyamides did not bind. Fluorescein isothiocyanate-labeled Py-Im polyamide was detected in the nuclei of human vascular smooth muscle cells (VSMCs) after 2- to 48-h incubation. Py-Im polyamide significantly decreased the promoter activity of hTGF-β1 determined by in vitro transcription experiments and luciferase assay. In cultured human VSMCs, Py-Im polyamide targeting hTGF-β1 promoter significantly inhibited expressions of hTGF-β1 mRNA and protein. These results indicate that the synthetic Py-Im polyamide designed to bind hTGF-β1 promoter inhibited hTGF-β1 gene and protein expression successfully. This novel agent will be used for the TGF-β-related diseases as a gene therapy.

Original languageEnglish
Pages (from-to)571-575
Number of pages5
JournalJournal of Pharmacology and Experimental Therapeutics
Issue number2
Publication statusPublished - Nov 2005


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