Structural analysis of an extracellular polysaccharide produced by a benzene tolerant bacterium, Rhodococcus sp. 33

Makoto Urai, Tomoko Aizawa, Hirosi Anzai, Jun Ogihara, Noriyuki Iwabuchi, Brett Neilan, Iain Couperwhite, Mutsuyasu Nakajima, Michio Sunairi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Rhodococcus sp. 33 can tolerate and efficiently degrade various concentrations of benzene, one of the most toxic and prevailing environmental pollutants. This strain produces a large quantity of extracellular polysaccharide (33 EPS), which plays an important role in the benzene tolerance in Rhodococcus sp. 33, especially by helping the cells to survive an initial challenge with benzene. This EPS has been reported to be composed of d-galactose, d-glucose, d-mannose, d-glucuronic acid, and pyruvic acid at a molar ratio of 1:1:1:1:1. To understand the protective effect of 33 EPS, we determined its chemical structure by using 1H and 13C NMR spectroscopy including 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units with the following structure: (Equation presented).

Original languageEnglish
Pages (from-to)616-623
Number of pages8
JournalCarbohydrate Research
Volume341
Issue number5
DOIs
Publication statusPublished - 10 Apr 2006

Keywords

  • Benzene tolerance
  • Bioremediation
  • Extracellular polysaccharide
  • NMR spectroscopy
  • Rhodococcus
  • Structure

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