TY - JOUR
T1 - Structural analysis of an extracellular polysaccharide produced by a benzene tolerant bacterium, Rhodococcus sp. 33
AU - Urai, Makoto
AU - Aizawa, Tomoko
AU - Anzai, Hirosi
AU - Ogihara, Jun
AU - Iwabuchi, Noriyuki
AU - Neilan, Brett
AU - Couperwhite, Iain
AU - Nakajima, Mutsuyasu
AU - Sunairi, Michio
PY - 2006/4/10
Y1 - 2006/4/10
N2 - Rhodococcus sp. 33 can tolerate and efficiently degrade various concentrations of benzene, one of the most toxic and prevailing environmental pollutants. This strain produces a large quantity of extracellular polysaccharide (33 EPS), which plays an important role in the benzene tolerance in Rhodococcus sp. 33, especially by helping the cells to survive an initial challenge with benzene. This EPS has been reported to be composed of d-galactose, d-glucose, d-mannose, d-glucuronic acid, and pyruvic acid at a molar ratio of 1:1:1:1:1. To understand the protective effect of 33 EPS, we determined its chemical structure by using 1H and 13C NMR spectroscopy including 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units with the following structure: (Equation presented).
AB - Rhodococcus sp. 33 can tolerate and efficiently degrade various concentrations of benzene, one of the most toxic and prevailing environmental pollutants. This strain produces a large quantity of extracellular polysaccharide (33 EPS), which plays an important role in the benzene tolerance in Rhodococcus sp. 33, especially by helping the cells to survive an initial challenge with benzene. This EPS has been reported to be composed of d-galactose, d-glucose, d-mannose, d-glucuronic acid, and pyruvic acid at a molar ratio of 1:1:1:1:1. To understand the protective effect of 33 EPS, we determined its chemical structure by using 1H and 13C NMR spectroscopy including 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units with the following structure: (Equation presented).
KW - Benzene tolerance
KW - Bioremediation
KW - Extracellular polysaccharide
KW - NMR spectroscopy
KW - Rhodococcus
KW - Structure
UR - http://www.scopus.com/inward/record.url?scp=33644631150&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2006.01.010
DO - 10.1016/j.carres.2006.01.010
M3 - Article
C2 - 16442512
AN - SCOPUS:33644631150
SN - 0008-6215
VL - 341
SP - 616
EP - 623
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 5
ER -