Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Amy Y. Hsiao; Teru Okitsu; Hiroaki Onoe; Mahiro Kiyosawa; Hiroki Teramae; Shintaroh Iwanaga; Shigenori Miura; Tomohiko Kazama; Taro Matsumoto; Shoji Takeuchi

doi:10.1109/MEMSYS.2013.6474179

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Shigenori Miura, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

Original language	English
Title of host publication	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages	71-73
Number of pages	3
DOIs	https://doi.org/10.1109/MEMSYS.2013.6474179
Publication status	Published - 2013
Event	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China Duration: 20 Jan 2013 → 24 Jan 2013

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Country/Territory	Taiwan, Province of China
City	Taipei
Period	20/01/13 → 24/01/13

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10.1109/MEMSYS.2013.6474179

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Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., Miura, S., Kazama, T., Matsumoto, T., & Takeuchi, S. (2013). Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 71-73). Article 6474179 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474179

@inproceedings{20931c96b3a0404fad6ec8a893df89a6,

title = "Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells",

abstract = "This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.",

author = "Hsiao, {Amy Y.} and Teru Okitsu and Hiroaki Onoe and Mahiro Kiyosawa and Hiroki Teramae and Shintaroh Iwanaga and Shigenori Miura and Tomohiko Kazama and Taro Matsumoto and Shoji Takeuchi",

year = "2013",

doi = "10.1109/MEMSYS.2013.6474179",

language = "English",

isbn = "9781467356558",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "71--73",

booktitle = "IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013",

note = "IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 ; Conference date: 20-01-2013 Through 24-01-2013",

}

Hsiao, AY, Okitsu, T, Onoe, H, Kiyosawa, M, Teramae, H, Iwanaga, S, Miura, S, Kazama, T, Matsumoto, T & Takeuchi, S 2013, Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474179, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 71-73, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 20/01/13. https://doi.org/10.1109/MEMSYS.2013.6474179

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. / Hsiao, Amy Y.; Okitsu, Teru; Onoe, Hiroaki et al.
IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 71-73 6474179 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

AU - Hsiao, Amy Y.

AU - Okitsu, Teru

AU - Onoe, Hiroaki

AU - Kiyosawa, Mahiro

AU - Teramae, Hiroki

AU - Iwanaga, Shintaroh

AU - Miura, Shigenori

AU - Kazama, Tomohiko

AU - Matsumoto, Taro

AU - Takeuchi, Shoji

PY - 2013

Y1 - 2013

N2 - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

AB - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

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U2 - 10.1109/MEMSYS.2013.6474179

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SN - 9781467356558

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

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Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S et al. Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 71-73. 6474179. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2013.6474179

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

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