Salivary alpha-amylase activity is increased by noxious stimuli under general anesthesia

  • Noriya Hirose
  • , Miki Matsui
  • , Masumi Itagaki
  • , Nao Shimizu
  • , Mizuki Mizoguchi
  • , Hisataka Kitano
  • , Emily Chang
  • , Kanako Yamagata
  • , Takahiro Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: This study evaluated changes in salivary alpha-amylase activity (sAA) before and after orotracheal intubation (TI) and precordial skin incision (SI) to investigate whether sAA was increased by noxious stimuli while under general anesthesia. Methods: sAA was measured before and after TI (study A: randomized double-blind test) and SI (study B; single-arm observational test) in 50 and 30 patients, respectively. Study A patients were divided into 2 groups of 25 each according to administration of 1.0 (LF group) or 2.0 µg/kg of fentanyl (HF group) during anesthesia induction. sAA was measured at four points, with study A measurements performed before anesthesia induction, immediately before TI, and at 3 and 6 min after TI, while study B measurements were performed before anesthesia induction, immediately before SI, and at 3 and 6 min after SI, respectively. Results: sAA significantly increased at 3 min after TI, and at 3 and 6 min after SI compared to before the procedure (P < 0.01). In study A, the observed sAA levels at 3 and 6 min after TI in the HF group were significantly lower than those observed in the LF group (P < 0.01). Conclusions: sAA increased after TI and SI. sAA measurements might be an ancillary indicator for sympathetic tone associated with noxious stimuli under general anesthesia.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalJournal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume38
Issue number2
DOIs
Publication statusPublished - Mar 2026

Keywords

  • General anesthesia
  • Noxious stimuli
  • Salivary alpha-amylase activity

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