S-genotype identification based on allele-specific PCR in Japanese pear

Kenji Nashima, Shingo Terakami, Sogo Nishio, Miyuki Kunihisa, Chikako Nishitani, Toshihiro Saito, Toshiya Yamamoto

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18 Citations (Scopus)


Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multiallelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S1-S9 and Sk) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S1/S1-S9/S9 and S4sm/S4sm) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalBreeding Science
Issue number3
Publication statusPublished - 2015
Externally publishedYes


  • Pyrus pyrifolia
  • S-RNase
  • S-genotyping
  • Self-incompatibility


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