Rapid removal of detergent in glycolipids using ionic liquids

Shoko Matsushita, Kazuhiko Noda, Shuta Hara, Jumpei Aisu, Aki Hayashi, Hiroki Ikake, Shigeru Shimizu, Minoru Tanigawa, Motohiko Ukiya, Katsushi Nishimura, Atsuyoshi Nishina, Yusuke Suzuki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Detergent removal in glycolipid after sample preparation, such as enzymatic reaction or isolation of detergent-resistant membrane microdomain, is indispensable for further structural characterization. We previously established the rapid and effective method of detergent removal in glycolipid samples from glass test tube using 1,2-dichloroethane (DCE) washing. However, the use of DCE has several drawbacks, such as environmental risks, harmful effects (potentially carcinogenic), and high vaporability and flammability. To solve the issue, we used ionic liquids to remove detergents from glycolipid samples, and found 1-butyl-3-methylimidazolium iodide was a suitable alternative for DCE. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1115-1121
Number of pages7
JournalAnalytical Sciences
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 2022

Keywords

  • 1,2-dichloroethane
  • Ganglioside
  • Glycosphingolipid
  • Ionic liquid
  • Thin-layer chromatography

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