TY - JOUR
T1 - Rapid and simple detection of Ureaplasma species from vaginal swab samples using a loop-mediated isothermal amplification method
AU - Fuwa, Kazumasa
AU - Seki, Mitsuko
AU - Hirata, Yoshiyasu
AU - Yanagihara, Itaru
AU - Nakura, Yukiko
AU - Takano, Chika
AU - Kuroda, Kazumichi
AU - Hayakawa, Satoshi
N1 - Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2018/1
Y1 - 2018/1
N2 - Problem: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. Method of study: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR. Results: Our LAMP primers were specific to each species and had no cross-reaction. Of 46 clinical specimens, the sensitivity, specificity, and positive and negative predictive values of the LAMP method were 100% (12/12), 100% (34/34), 100% (12/12), and 100% (34/34), respectively, whereas those of PCR were 66.7% (8/12), 100% (34/34), 100% (8/8), and 89.5% (34/38), respectively, compared to culture-based detection. Conclusion: The LAMP detection method outperformed the culture and PCR methods. Early detection enables appropriate antibiotic selection for improved prenatal outcomes.
AB - Problem: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. Method of study: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR. Results: Our LAMP primers were specific to each species and had no cross-reaction. Of 46 clinical specimens, the sensitivity, specificity, and positive and negative predictive values of the LAMP method were 100% (12/12), 100% (34/34), 100% (12/12), and 100% (34/34), respectively, whereas those of PCR were 66.7% (8/12), 100% (34/34), 100% (8/8), and 89.5% (34/38), respectively, compared to culture-based detection. Conclusion: The LAMP detection method outperformed the culture and PCR methods. Early detection enables appropriate antibiotic selection for improved prenatal outcomes.
KW - LAMP method
KW - Ureaplasma species
KW - Vaginal sample
UR - http://www.scopus.com/inward/record.url?scp=85034612763&partnerID=8YFLogxK
U2 - 10.1111/aji.12771
DO - 10.1111/aji.12771
M3 - Article
C2 - 29154392
AN - SCOPUS:85034612763
SN - 1046-7408
VL - 79
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 1
M1 - e12771
ER -