Quantitative evaluation of MDP-Ca salt and DCPD after application of an MDP-based one-step self-etching adhesive on enamel and dentin

Yoko Yokota, Kou Nakajima Fujita, Ryoichiro Uchida, Etsuko Aida, Naoko Tabei Aoki, Masahiro Aida, Norihiro Nishiyama

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Purpose: To investigate the effects of an experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based one-step self-etching adhesive (EX adhesive) applied to enamel and dentin on the production of calcium salt of MDP (MDP-Ca salt) and dicalcium phosphate dehydrate (DCPD) at various periods. Materials and Methods: The EX adhesive was prepared. Bovine enamel and dentin reactants were prepared by varying the application period of the EX adhesive: 0.5, 1, 5, 30, 60 and 1440 min. Enamel and dentin reactants were analyzed using x-ray diffraction and solid-state phosphorus-31 nuclear magnetic resonance (31P NMR). Curvefitting analyses of corresponding 31P NMR spectra were performed. Results: Enamel and dentin developed several types of MDP-Ca salts and DCPDs with amorphous and crystalline phases throughout the application period. The predominant molecular species of MDP-Ca salt was determined as the monocalcium salt of the MDP monomer. Dentin showed a faster production rate and greater produced amounts of MDP-Ca salt than did enamel, since enamel showed a knee-point in the production rate of the MDP-Ca salt at the application period of 5 min. In contrast, enamel developed greater amounts of DCPD than did dentin and two types of DCPDs with different crystalline phases at application periods > 30 min. The amounts of MDP-Ca salt developed during the 30-s application of the EX adhesive on enamel and dentin were 7.3 times and 21.2 times greater than DCPD, respectively. Conclusion: The MDP-based one-step adhesive yielded several types of MDP-Ca salts and DCPD with an amorphous phase during the 30-s application period on enamel and dentin.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalJournal of Adhesive Dentistry
Issue number3
Publication statusPublished - 2016


  • Calcium salt of MDP
  • MDP
  • Nuclear magnetic resonance
  • One-step self-etching adhesive
  • X-ray diffraction


Dive into the research topics of 'Quantitative evaluation of MDP-Ca salt and DCPD after application of an MDP-based one-step self-etching adhesive on enamel and dentin'. Together they form a unique fingerprint.

Cite this