Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)

Takahiro Ogura; Jun Ogihara; Michio Sunairi; Hidetaka Takeishi; Tomoko Aizawa; Marcos R. Olivos-Trujillo; Iván J. Maureira-Butler; Haroldo E. Salvo-Garrido

doi:10.1002/pmic.201300511

Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)

Takahiro Ogura, Jun Ogihara, Michio Sunairi, Hidetaka Takeishi, Tomoko Aizawa, Marcos R. Olivos-Trujillo, Iván J. Maureira-Butler, Haroldo E. Salvo-Garrido

College of Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).

Original language	English
Pages (from-to)	1543-1546
Number of pages	4
Journal	Proteomics
Volume	14
Issue number	12
DOIs	https://doi.org/10.1002/pmic.201300511
Publication status	Published - Jun 2014

Keywords

2DE
Legume seed
Lupinus luteus L
Nano-LC-MS/MS
Plant proteomics

Access to Document

10.1002/pmic.201300511

Cite this

@article{4cef4ce85ea3480a8ac5ecb76544a319,

title = "Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)",

abstract = "Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).",

keywords = "2DE, Legume seed, Lupinus luteus L, Nano-LC-MS/MS, Plant proteomics",

author = "Takahiro Ogura and Jun Ogihara and Michio Sunairi and Hidetaka Takeishi and Tomoko Aizawa and Olivos-Trujillo, {Marcos R.} and Maureira-Butler, {Iv{\'a}n J.} and Salvo-Garrido, {Haroldo E.}",

year = "2014",

month = jun,

doi = "10.1002/pmic.201300511",

language = "English",

volume = "14",

pages = "1543--1546",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "12",

}

TY - JOUR

T1 - Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)

AU - Ogura, Takahiro

AU - Ogihara, Jun

AU - Sunairi, Michio

AU - Takeishi, Hidetaka

AU - Aizawa, Tomoko

AU - Olivos-Trujillo, Marcos R.

AU - Maureira-Butler, Iván J.

AU - Salvo-Garrido, Haroldo E.

PY - 2014/6

Y1 - 2014/6

N2 - Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).

AB - Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).

KW - 2DE

KW - Legume seed

KW - Lupinus luteus L

KW - Nano-LC-MS/MS

KW - Plant proteomics

UR - http://www.scopus.com/inward/record.url?scp=84902171153&partnerID=8YFLogxK

U2 - 10.1002/pmic.201300511

DO - 10.1002/pmic.201300511

M3 - Article

C2 - 24723484

AN - SCOPUS:84902171153

SN - 1615-9853

VL - 14

SP - 1543

EP - 1546

JO - Proteomics

JF - Proteomics

IS - 12

ER -

Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this