Periodontal tissue regeneration by transplantation of rat adipose-derived stromal cells in combination with PLGA-based solid scaffolds

Daisuke Akita, Masakazu Morokuma, Yoko Saito, Katsuyuki Yamanaka, Yuko Akiyama, Momoko Sato, Takayuki Mashimo, Taku Toriumi, Yoshinori Arai, Tadashi Kaneko, Naoki Tsukimura, Keitaro Isokawa, Tomohiko Ishigami, Masaki J. Honda

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Regeneration of damaged periodontium is challenging due to its multi-tissue composition. Mesenchymal stem cell-based approaches using adipose-derived stromal cells (ASCs) may contribute to periodontal reconstruction, particularly when combined with the use of scaffolds to maintain a space for new tissue growth. The aim of this study was to assess the regenerative potential of ASCs derived from inbred or outbred rats in combination with novel solid scaffolds composed of PLGA (Poly d,l-lactic-co-glycolic acid) (PLGA-scaffolds). Cultured ASCs seeded onto PLGAscaffolds (ASCs/PLGA) or PLGA-scaffolds (PLGA) alone were transplanted into periodontal fenestration defects created in F344 or Sprague Dawley (SD) rats. Micro-CT analysis showed a significantly higher percentage of bone growth in the ASCs/PLGA groups compared with the PLGA-alone groups at five weeks after surgery. Similarly, histomorphometric analysis demonstrated thicker growth of periodontal ligament and cementum layers in the ASCs/PLGA-groups compared with the PLGA-alone groups. In addition, transplanted DiI-labeled ASCs were observed in the periodontal regenerative sites. The present investigation demonstrated the marked ability of ASCs in combination with PLGA scaffolds to repair periodontal defects.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalBiomedical Research
Volume35
Issue number2
DOIs
Publication statusPublished - 2014

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