TY - JOUR
T1 - Oral administration of geranylgeraniol rescues denervationinduced muscle atrophy via suppression of Atrogin-1
AU - Miyawaki, Aki
AU - Rojasawasthien, Thira
AU - Hitomi, Suzuro
AU - Aoki, Yoshinori
AU - Urata, Mariko
AU - Inoue, Asako
AU - Matsubara, Takuma
AU - Morikawa, Kazumasa
AU - Habu, Manabu
AU - Tominaga, Kazuhiro
AU - Kokabu, Shoichiro
N1 - Publisher Copyright:
© 2020 International Institute of Anticancer Research. All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - Background/Aim: Geranylgeraniol (GGOH), a C20 isoprenoid naturally occurs in several foods. We previously reported that GGOH treatment reduced the expression levels of Atrogin-1 which is involved in skeletal muscle degradation and stimulates the myogenic differentiation of C2C12 myoblasts. However, the effect of GGOH supplementation on skeletal muscle metabolism in vivo is unknown. Materials and Methods: Skeletal muscle atrophy was induced by denervation. The expression levels of Atrogin-1 were assessed by western blotting or real time PCR. Results: Intraoral administration of GGOH reduced the decrease in the cross-sectional area of muscle fibers and also suppressed the expression levels of Atrogin-1 in denervation induced muscle atrophy. Also, GGOH treatment suppressed the expression of Atrogin-1 and the decrease in skeletal muscle fiber size by glucocorticoid in vitro. Conclusion: Intraoral administration of GGOH rescues denervation-induced muscle atrophy via suppression of Atrogin-1.
AB - Background/Aim: Geranylgeraniol (GGOH), a C20 isoprenoid naturally occurs in several foods. We previously reported that GGOH treatment reduced the expression levels of Atrogin-1 which is involved in skeletal muscle degradation and stimulates the myogenic differentiation of C2C12 myoblasts. However, the effect of GGOH supplementation on skeletal muscle metabolism in vivo is unknown. Materials and Methods: Skeletal muscle atrophy was induced by denervation. The expression levels of Atrogin-1 were assessed by western blotting or real time PCR. Results: Intraoral administration of GGOH reduced the decrease in the cross-sectional area of muscle fibers and also suppressed the expression levels of Atrogin-1 in denervation induced muscle atrophy. Also, GGOH treatment suppressed the expression of Atrogin-1 and the decrease in skeletal muscle fiber size by glucocorticoid in vitro. Conclusion: Intraoral administration of GGOH rescues denervation-induced muscle atrophy via suppression of Atrogin-1.
KW - Atrogin-1
KW - Geranylgeraniol
KW - Skeletal muscle atrophy
UR - http://www.scopus.com/inward/record.url?scp=85090180492&partnerID=8YFLogxK
U2 - 10.21873/invivo.12047
DO - 10.21873/invivo.12047
M3 - Article
C2 - 32871759
AN - SCOPUS:85090180492
SN - 1791-7549
VL - 34
SP - 2345
EP - 2351
JO - In vivo (Athens, Greece)
JF - In vivo (Athens, Greece)
IS - 5
ER -
