TY - JOUR
T1 - Optimization and validation of a high-performance liquid chromatographic method with UV detection for the determination of pyrrole-imidazole polyamides in rat plasma
AU - Fukasawa, Akiko
AU - Nagashima, Takashi
AU - Aoyama, Takahiko
AU - Fukuda, Noboru
AU - Matsuda, Hiroyuki
AU - Ueno, Takahiro
AU - Sugiyama, Hiroshi
AU - Nagase, Hiroki
AU - Matsumoto, Yoshiaki
PY - 2007/11/15
Y1 - 2007/11/15
N2 - A simple and sensitive high-performance liquid chromatography (HPLC) method utilizing UV detection was developed for the determination of plasma pyrrole (Py)-imidazole (Im) polyamides in rats and applied to the pharmacokinetic study of compounds. After deproteinization of plasma with methanol, Py-Im polyamides were analyzed with a reversed-phase TSK-GEL ODS-80TM (4.6 mm × 15.0 cm TOSOH Co., Japan) column maintained at 40 °C. The mobile phase solvent A was 0.1% acetic acid and the solvent B was HPLC-grade acetonitrile (0-10 min, A: 100-20%, B: 0-80% linear gradient; 10-15 min, A: 40%, B: 60%). The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. The method was used to determine the plasma concentration time profiles of Py-Im polyamides after intravenous injection.
AB - A simple and sensitive high-performance liquid chromatography (HPLC) method utilizing UV detection was developed for the determination of plasma pyrrole (Py)-imidazole (Im) polyamides in rats and applied to the pharmacokinetic study of compounds. After deproteinization of plasma with methanol, Py-Im polyamides were analyzed with a reversed-phase TSK-GEL ODS-80TM (4.6 mm × 15.0 cm TOSOH Co., Japan) column maintained at 40 °C. The mobile phase solvent A was 0.1% acetic acid and the solvent B was HPLC-grade acetonitrile (0-10 min, A: 100-20%, B: 0-80% linear gradient; 10-15 min, A: 40%, B: 60%). The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. The method was used to determine the plasma concentration time profiles of Py-Im polyamides after intravenous injection.
KW - HPLC
KW - Pharmacokinetics
KW - Pyrrole-imidazole polyamide
KW - Rat plasma
UR - http://www.scopus.com/inward/record.url?scp=35748938461&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2007.09.032
DO - 10.1016/j.jchromb.2007.09.032
M3 - Article
C2 - 17950049
AN - SCOPUS:35748938461
SN - 1570-0232
VL - 859
SP - 272
EP - 275
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -