Abstract
A simple and sensitive high-performance liquid chromatography (HPLC) method utilizing UV detection was developed for the determination of plasma pyrrole (Py)-imidazole (Im) polyamides in rats and applied to the pharmacokinetic study of compounds. After deproteinization of plasma with methanol, Py-Im polyamides were analyzed with a reversed-phase TSK-GEL ODS-80TM (4.6 mm × 15.0 cm TOSOH Co., Japan) column maintained at 40 °C. The mobile phase solvent A was 0.1% acetic acid and the solvent B was HPLC-grade acetonitrile (0-10 min, A: 100-20%, B: 0-80% linear gradient; 10-15 min, A: 40%, B: 60%). The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. The method was used to determine the plasma concentration time profiles of Py-Im polyamides after intravenous injection.
Original language | English |
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Pages (from-to) | 272-275 |
Number of pages | 4 |
Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 859 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Nov 2007 |
Keywords
- HPLC
- Pharmacokinetics
- Pyrrole-imidazole polyamide
- Rat plasma