No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

Meripet Polat Yamanaka; Susumu Saito; Yukiko Hara; Ryosuke Matsuura; Shin nosuke Takeshima; Kazuyoshi Hosomichi; Yasunobu Matsumoto; Rika A. Furuta; Masami Takei; Yoko Aida

doi:10.1186/s12977-022-00592-6

No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

Meripet Polat Yamanaka, Susumu Saito, Yukiko Hara, Ryosuke Matsuura, Shin nosuke Takeshima, Kazuyoshi Hosomichi, Yasunobu Matsumoto, Rika A. Furuta, Masami Takei, Yoko Aida

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. Result: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Conclusion: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.

Original language	English
Article number	7
Journal	Retrovirology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12977-022-00592-6
Publication status	Published - Dec 2022

Keywords

Antibody detection
Bovine leukemia virus (BLV)
Japanese breast cancers
Japanese human blood
Japanese human sera
PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/s12977-022-00592-6

Cite this

@article{91f9051dfcfa46ba86df90208709f28f,

title = "No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan",

abstract = "Background: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. Result: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Conclusion: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.",

keywords = "Antibody detection, Bovine leukemia virus (BLV), Japanese breast cancers, Japanese human blood, Japanese human sera, PCR",

author = "Yamanaka, {Meripet Polat} and Susumu Saito and Yukiko Hara and Ryosuke Matsuura and Takeshima, {Shin nosuke} and Kazuyoshi Hosomichi and Yasunobu Matsumoto and Furuta, {Rika A.} and Masami Takei and Yoko Aida",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1186/s12977-022-00592-6",

language = "English",

volume = "19",

journal = "Retrovirology",

issn = "1742-4690",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

AU - Yamanaka, Meripet Polat

AU - Saito, Susumu

AU - Hara, Yukiko

AU - Matsuura, Ryosuke

AU - Takeshima, Shin nosuke

AU - Hosomichi, Kazuyoshi

AU - Matsumoto, Yasunobu

AU - Furuta, Rika A.

AU - Takei, Masami

AU - Aida, Yoko

PY - 2022/12

Y1 - 2022/12

N2 - Background: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. Result: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Conclusion: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.

AB - Background: The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential. Result: In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples. Conclusion: Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.

KW - Antibody detection

KW - Bovine leukemia virus (BLV)

KW - Japanese breast cancers

KW - Japanese human blood

KW - Japanese human sera

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=85130214040&partnerID=8YFLogxK

U2 - 10.1186/s12977-022-00592-6

DO - 10.1186/s12977-022-00592-6

M3 - Article

C2 - 35585539

AN - SCOPUS:85130214040

SN - 1742-4690

VL - 19

JO - Retrovirology

JF - Retrovirology

IS - 1

M1 - 7

ER -

No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this