Mucilaginibacter kameinonensis sp., nov., isolated from garden soil

Makoto Urai, Tomoko Aizawa, Yasuyoshi Nakagawa, Mutsuyasu Nakajima, Michio Sunairi

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

An extracellular polysaccharide-producing bacterium, strain SCKT, was isolated from a soil sample taken from Kameino, Fujisawa, Japan. The isolate was Gram-negative and cells were non-motile, irregular-shaped rods that grew optimally at 25 °C and grew between pH 5 and 8. Strain SCKT contained MK-7 as the major isoprenoid quinone, iso-C15 : 0 and C16 : 1ω7c and/or iso-C15 : 0 2-OH as the major fatty acids and sphingolipids, with d-17 : 0 as the main dihydrosphingosine. Flexirubin-type pigments were also present. The DNA G+C content was 43.7 mol%. On the basis of 16S rRNA gene sequence analysis, strain SCKT was shown to belong to the genus Mucilaginibacter. The 16S rRNA gene sequence similarity between strain SCKT and the two type strains of Mucilaginibacter was 93 %. The results of physiological and biochemical tests allowed phenotypic differentiation of the strain from published Mucilaginibacter species. Therefore, strain SCKT represents a novel species, for which the name Mucilaginibacter kameinonensis sp. nov. is proposed. The type strain is SCKT (=NBRC 102645T 5KCTC 22227T).

Original languageEnglish
Pages (from-to)2046-2050
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number9
DOIs
Publication statusPublished - Sept 2008

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