Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel

Rui Kano; Yumi Sakamoto; Ako Hanahachi; Hiroshi Kamata; Youichi Fukuda; Kosaku Fujiwara; Atsuhiko Hasegawa

doi:10.1177/104063870101300515

Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel

Rui Kano, Yumi Sakamoto, Ako Hanahachi, Hiroshi Kamata, Youichi Fukuda, Kosaku Fujiwara, Atsuhiko Hasegawa

College of Bioresource Sciences

Nihon University

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Candida spp. Polymerase chain reaction amplification of DNA from crop lesion material with internal transcribed spacer 2 (ITS2) primers yielded fragments of approximately 300 bp, which demonstrated 95% DNA homology with the corresponding sequence from a strain of Candida parapsilosis deposited in the GenBank data base. The Candida species in the lesion of the crop mucosa was therefore identified by DNA sequence analysis as C. parapsilosis.

Original language	English
Pages (from-to)	437-439
Number of pages	3
Journal	Journal of Veterinary Diagnostic Investigation
Volume	13
Issue number	5
DOIs	https://doi.org/10.1177/104063870101300515
Publication status	Published - 2001

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abstract = "A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Candida spp. Polymerase chain reaction amplification of DNA from crop lesion material with internal transcribed spacer 2 (ITS2) primers yielded fragments of approximately 300 bp, which demonstrated 95% DNA homology with the corresponding sequence from a strain of Candida parapsilosis deposited in the GenBank data base. The Candida species in the lesion of the crop mucosa was therefore identified by DNA sequence analysis as C. parapsilosis.",

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AU - Kano, Rui

AU - Sakamoto, Yumi

AU - Hanahachi, Ako

AU - Kamata, Hiroshi

AU - Fukuda, Youichi

AU - Fujiwara, Kosaku

AU - Hasegawa, Atsuhiko

PY - 2001

Y1 - 2001

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AB - A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Candida spp. Polymerase chain reaction amplification of DNA from crop lesion material with internal transcribed spacer 2 (ITS2) primers yielded fragments of approximately 300 bp, which demonstrated 95% DNA homology with the corresponding sequence from a strain of Candida parapsilosis deposited in the GenBank data base. The Candida species in the lesion of the crop mucosa was therefore identified by DNA sequence analysis as C. parapsilosis.

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Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel

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