Molecular characterization and immune responsive expression of feline MDA5 gene

Ryota Koba; Sakurako Shoji; Sawami Yoshimura; Yukinobu Tohya

doi:10.1292/jvms.17-0278

Molecular characterization and immune responsive expression of feline MDA5 gene

Ryota Koba, Sakurako Shoji, Sawami Yoshimura, Yukinobu Tohya

College of Bioresource Sciences

Nihon University

Research output: Contribution to journal › Comment/debate

Abstract

The retinoic acid-inducible gene-I-like receptor (RLR) family is a group of cytosolic RNA helicase proteins that play an important role in sensing viral RNAs. Melanoma differentiation-associated gene 5 (MDA5), an RLR protein, recognizes viral double-stranded RNA and 5’-triphosphate single-stranded RNA in the cytoplasm for the expression of type I interferon (IFN). The expression of MDA5 is also induced by type I IFN. In the present study, we determined the complete coding sequence of the feline MDA5 gene, and analyzed its structure. In addition, we examined tissue expression patterns, inducibilities of the feline MDA5 by polyinosinic-polycytidylic acid and type I IFN, and a functional role of feline MDA5 on type I IFN expression.

Original language	English
Pages (from-to)	1266-1270
Number of pages	5
Journal	Journal of Veterinary Medical Science
Volume	80
Issue number	8
DOIs	https://doi.org/10.1292/jvms.17-0278
Publication status	Published - Aug 2018

Keywords

Cat
Innate immunity
Interferon
Melanoma differentiation-associated gene 5 (MDA5)

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10.1292/jvms.17-0278

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title = "Molecular characterization and immune responsive expression of feline MDA5 gene",

abstract = "The retinoic acid-inducible gene-I-like receptor (RLR) family is a group of cytosolic RNA helicase proteins that play an important role in sensing viral RNAs. Melanoma differentiation-associated gene 5 (MDA5), an RLR protein, recognizes viral double-stranded RNA and 5{\textquoteright}-triphosphate single-stranded RNA in the cytoplasm for the expression of type I interferon (IFN). The expression of MDA5 is also induced by type I IFN. In the present study, we determined the complete coding sequence of the feline MDA5 gene, and analyzed its structure. In addition, we examined tissue expression patterns, inducibilities of the feline MDA5 by polyinosinic-polycytidylic acid and type I IFN, and a functional role of feline MDA5 on type I IFN expression.",

keywords = "Cat, Innate immunity, Interferon, Melanoma differentiation-associated gene 5 (MDA5)",

author = "Ryota Koba and Sakurako Shoji and Sawami Yoshimura and Yukinobu Tohya",

note = "Publisher Copyright: {\textcopyright}2018 The Japanese Society of Veterinary Science.",

year = "2018",

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doi = "10.1292/jvms.17-0278",

language = "English",

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journal = "Journal of Veterinary Medical Science",

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T1 - Molecular characterization and immune responsive expression of feline MDA5 gene

AU - Koba, Ryota

AU - Shoji, Sakurako

AU - Yoshimura, Sawami

AU - Tohya, Yukinobu

PY - 2018/8

Y1 - 2018/8

N2 - The retinoic acid-inducible gene-I-like receptor (RLR) family is a group of cytosolic RNA helicase proteins that play an important role in sensing viral RNAs. Melanoma differentiation-associated gene 5 (MDA5), an RLR protein, recognizes viral double-stranded RNA and 5’-triphosphate single-stranded RNA in the cytoplasm for the expression of type I interferon (IFN). The expression of MDA5 is also induced by type I IFN. In the present study, we determined the complete coding sequence of the feline MDA5 gene, and analyzed its structure. In addition, we examined tissue expression patterns, inducibilities of the feline MDA5 by polyinosinic-polycytidylic acid and type I IFN, and a functional role of feline MDA5 on type I IFN expression.

AB - The retinoic acid-inducible gene-I-like receptor (RLR) family is a group of cytosolic RNA helicase proteins that play an important role in sensing viral RNAs. Melanoma differentiation-associated gene 5 (MDA5), an RLR protein, recognizes viral double-stranded RNA and 5’-triphosphate single-stranded RNA in the cytoplasm for the expression of type I interferon (IFN). The expression of MDA5 is also induced by type I IFN. In the present study, we determined the complete coding sequence of the feline MDA5 gene, and analyzed its structure. In addition, we examined tissue expression patterns, inducibilities of the feline MDA5 by polyinosinic-polycytidylic acid and type I IFN, and a functional role of feline MDA5 on type I IFN expression.

KW - Cat

KW - Innate immunity

KW - Interferon

KW - Melanoma differentiation-associated gene 5 (MDA5)

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U2 - 10.1292/jvms.17-0278

DO - 10.1292/jvms.17-0278

M3 - Comment/debate

C2 - 29973480

AN - SCOPUS:85052551832

SN - 0916-7250

VL - 80

SP - 1266

EP - 1270

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 8

ER -

Molecular characterization and immune responsive expression of feline MDA5 gene

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