Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro

Tomohiko Kazama, Masaki Fujie, Tuyoshi Endo, Koichiro Kano

Research output: Contribution to journalArticlepeer-review

84 Citations (Scopus)

Abstract

We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into osteoblasts and chondrocytes under appropriate culture conditions. However, it is unclear whether DFAT cells are capable of transdifferentiating into skeletal myocytes, which is common in the mesodermal lineage. Here, we show that DFAT cells can be induced to transdifferentiate into skeletal myocytes in vitro. Myogenic induction of DFAT cells resulted in the expression of MyoD and myogenin, followed by cell fusion and formation of multinucleated cells expressing sarcomeric myosin heavy chain. These results indicate that DFAT cells derived from mature adipocytes can transdifferentiate into skeletal myocytes in vitro.

Original languageEnglish
Pages (from-to)780-785
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number3
DOIs
Publication statusPublished - 19 Dec 2008

Keywords

  • 5-Azacytidine
  • Adipocyte
  • Dedifferentiation
  • Epigenetics
  • Methylation
  • MyoD
  • Myocyte
  • Myogenin
  • Myotube
  • Transdifferentiation

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