TY - JOUR
T1 - Lower expressions of the human bitter taste receptor TAS2R in smokers
T2 - Reverse transcriptase-polymerase chain reaction analysis
AU - Aoki, Mieko
AU - Takao, Tetsuya
AU - Takao, Kyoichi
AU - Koike, Fumihiko
AU - Suganuma, Narufumi
N1 - Publisher Copyright:
© 2014 Aoki et al.; licensee BioMed Central Ltd.
PY - 2014/8/15
Y1 - 2014/8/15
N2 - Background: Despite the fact that smokers have deficit in detecting taste, particularly bitter taste, no study has investigated its biological correlate. Methods. In this context, we compared the expression of the bitter taste receptor gene, taste 2 receptor (TAS2R) in the tongues of smokers and non-smokers. Tissue samples were collected from the lateral portion of the tongues of 22 smokers and 22 age- and gender-matched healthy volunteers (19 males and three females) with no history of smoking. Reverse transcriptase-polymerase chain reaction was used to examine the expression of TAS2R in the two groups, and the effect of aging on TAS2R expression was also assessed. Results: TAS2R expression was significantly lower among smokers than non-smokers (t = 6.525, P <.0001, 11.36 ± 6.0 vs. 2.09 ± 2.8, mean ± SD, non-smokers vs. smokers). Further, a positive correlation between age and expression of TAS2R was observed in non-smokers (r =.642, P =.001), but not smokers (r =.124, P =.584). This correlation difference was significant (Z = 1.96, P =.0496). Conclusions: Smokers showed a significantly lower expression of the bitter taste receptor gene than non-smokers, which is potentially caused by their inability to acquire such receptors with age because of cigarette smoking, in contrast to non-smokers.
AB - Background: Despite the fact that smokers have deficit in detecting taste, particularly bitter taste, no study has investigated its biological correlate. Methods. In this context, we compared the expression of the bitter taste receptor gene, taste 2 receptor (TAS2R) in the tongues of smokers and non-smokers. Tissue samples were collected from the lateral portion of the tongues of 22 smokers and 22 age- and gender-matched healthy volunteers (19 males and three females) with no history of smoking. Reverse transcriptase-polymerase chain reaction was used to examine the expression of TAS2R in the two groups, and the effect of aging on TAS2R expression was also assessed. Results: TAS2R expression was significantly lower among smokers than non-smokers (t = 6.525, P <.0001, 11.36 ± 6.0 vs. 2.09 ± 2.8, mean ± SD, non-smokers vs. smokers). Further, a positive correlation between age and expression of TAS2R was observed in non-smokers (r =.642, P =.001), but not smokers (r =.124, P =.584). This correlation difference was significant (Z = 1.96, P =.0496). Conclusions: Smokers showed a significantly lower expression of the bitter taste receptor gene than non-smokers, which is potentially caused by their inability to acquire such receptors with age because of cigarette smoking, in contrast to non-smokers.
KW - Cigarette
KW - Human
KW - RT-PCR
KW - TAS2R
UR - http://www.scopus.com/inward/record.url?scp=84906923485&partnerID=8YFLogxK
U2 - 10.1186/1617-9625-12-12
DO - 10.1186/1617-9625-12-12
M3 - Article
AN - SCOPUS:84906923485
SN - 1617-9625
VL - 12
JO - Tobacco Induced Diseases
JF - Tobacco Induced Diseases
IS - 1
M1 - 12
ER -