Low Susceptibility of Rubella Virus in First-Trimester Trophoblast Cell Lines

Ngan Thi Kim Pham, Quang Duy Trinh, Kazuhide Takada, Shihoko Komine-Aizawa, Satoshi Hayakawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We recently published an article about myelin oligodendrocyte glycoprotein-independent rubella infection of keratinocytes in vitro, in which first-trimester trophoblast cells were shown as rubella virus (RuV)-resistant. Given an incident rate as high as 90% of congenital rubella syndrome in the first eight weeks of pregnancy, the RuV infection of first-trimester trophoblasts is considered key to opening the gate to transplacental transmission mechanisms. Therefore, with this study, we aimed to verify the susceptibility/resistance of first-trimester trophoblast cell lines, HTR-8/SVneo and Swan.71, against RuV. Cells cultured on multi-well plates were challenged with a RuV clinical strain at a multiplicity of infection from 5 to 10 for 3 h. The infectivity was investigated by immuno-fluorescence (IF) assay and flow cytometry (FCM) analysis. Supernatants collected during the post-infection period were used to determine virus-progeny production. The scattered signaling of RuV infection of these cells was noted by IF assay, and the FCM analysis showed an average of 4–5% of gated cells infected with RuV. In addition, a small but significant production of virus progeny was also observed. In conclusion, by employing appropriate approaches, we determined the low infec-tivity of RuV in first-trimester trophoblast cell lines but not resistance as in our previous report.

Original languageEnglish
Article number1169
Issue number6
Publication statusPublished - Jun 2022


  • correction
  • first trimester
  • flow cytometry
  • HTR-8/SVneo
  • resistance
  • rubella
  • susceptibility
  • Swan.71
  • trophoblast


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