Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

Soichiro Manaka; Natsuko Tanabe; Taro Kariya; Masako Naito; Tadahiro Takayama; Mayu Nagao; Di Liu; Koichi Ito; Masao Maeno; Naoto Suzuki; Masashi Miyazaki

doi:10.1016/j.febslet.2014.12.013

Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

Soichiro Manaka, Natsuko Tanabe, Taro Kariya, Masako Naito, Tadahiro Takayama, Mayu Nagao, Di Liu, Koichi Ito, Masao Maeno, Naoto Suzuki, Masashi Miyazaki

School of Dentistry

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

Original language	English
Pages (from-to)	310-318
Number of pages	9
Journal	FEBS Letters
Volume	589
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2014.12.013
Publication status	Published - 30 Jan 2015

Keywords

Adenosine triphosphate
Low-intensity pulsed ultrasound
Osteogenesis
P2X7

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10.1016/j.febslet.2014.12.013

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title = "Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells",

abstract = "Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.",

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AU - Manaka, Soichiro

AU - Tanabe, Natsuko

AU - Kariya, Taro

AU - Naito, Masako

AU - Takayama, Tadahiro

AU - Nagao, Mayu

AU - Liu, Di

AU - Ito, Koichi

AU - Maeno, Masao

AU - Suzuki, Naoto

AU - Miyazaki, Masashi

PY - 2015/1/30

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N2 - Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

AB - Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

KW - Adenosine triphosphate

KW - Low-intensity pulsed ultrasound

KW - Osteogenesis

KW - P2X7

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Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

Abstract

Keywords

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