Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif

Masashi Taniguchi; Kyoko Fujiwara; Yuji Nakai; Toshinori Ozaki; Nobuko Koshikawa; Kojima Toshio; Motoaki Kataba; Asako Oguni; Hiroyuki Matsuda; Yukihiro Yoshida; Yasuaki Tokuhashi; Noboru Fukuda; Takahiro Ueno; Masayoshi Soma; Hiroki Nagase

doi:10.1016/j.fob.2014.03.004

Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif

Masashi Taniguchi, Kyoko Fujiwara, Yuji Nakai, Toshinori Ozaki, Nobuko Koshikawa, Kojima Toshio, Motoaki Kataba, Asako Oguni, Hiroyuki Matsuda, Yukihiro Yoshida, Yasuaki Tokuhashi, Noboru Fukuda, Takahiro Ueno, Masayoshi Soma, Hiroki Nagase

School of Medicine

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Gene amplification and/or overexpression of the transcription factor c-MYC, which binds to the E-box sequence (5'-CACGTG-3'), has been observed in many human tumors. In this study, we have designed 5 pyrrole-imidazole (PI) polyamides recognizing E-box, and found that, among them, Myc-6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc-6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc-6 exerts its tumor-suppressive ability at least in part through the specific down-regulation of MALAT1.

Original language	English
Pages (from-to)	328-334
Number of pages	7
Journal	FEBS Open Bio
Volume	4
DOIs	https://doi.org/10.1016/j.fob.2014.03.004
Publication status	Published - 2014

Keywords

E-box
MALAT1
MG63 cells
Osteosarcoma
Pyrrole-imidazole polyamide
c-MYC

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10.1016/j.fob.2014.03.004

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Taniguchi, M., Fujiwara, K., Nakai, Y., Ozaki, T., Koshikawa, N., Toshio, K., Kataba, M., Oguni, A., Matsuda, H., Yoshida, Y., Tokuhashi, Y., Fukuda, N., Ueno, T., Soma, M., & Nagase, H. (2014). Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif. FEBS Open Bio, 4, 328-334. https://doi.org/10.1016/j.fob.2014.03.004

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title = "Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif",

abstract = "Gene amplification and/or overexpression of the transcription factor c-MYC, which binds to the E-box sequence (5'-CACGTG-3'), has been observed in many human tumors. In this study, we have designed 5 pyrrole-imidazole (PI) polyamides recognizing E-box, and found that, among them, Myc-6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc-6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc-6 exerts its tumor-suppressive ability at least in part through the specific down-regulation of MALAT1.",

keywords = "E-box, MALAT1, MG63 cells, Osteosarcoma, Pyrrole-imidazole polyamide, c-MYC",

author = "Masashi Taniguchi and Kyoko Fujiwara and Yuji Nakai and Toshinori Ozaki and Nobuko Koshikawa and Kojima Toshio and Motoaki Kataba and Asako Oguni and Hiroyuki Matsuda and Yukihiro Yoshida and Yasuaki Tokuhashi and Noboru Fukuda and Takahiro Ueno and Masayoshi Soma and Hiroki Nagase",

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Taniguchi, M, Fujiwara, K, Nakai, Y, Ozaki, T, Koshikawa, N, Toshio, K, Kataba, M, Oguni, A, Matsuda, H, Yoshida, Y, Tokuhashi, Y, Fukuda, N, Ueno, T, Soma, M & Nagase, H 2014, 'Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif', FEBS Open Bio, vol. 4, pp. 328-334. https://doi.org/10.1016/j.fob.2014.03.004

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AU - Taniguchi, Masashi

AU - Fujiwara, Kyoko

AU - Nakai, Yuji

AU - Ozaki, Toshinori

AU - Koshikawa, Nobuko

AU - Toshio, Kojima

AU - Kataba, Motoaki

AU - Oguni, Asako

AU - Matsuda, Hiroyuki

AU - Yoshida, Yukihiro

AU - Tokuhashi, Yasuaki

AU - Fukuda, Noboru

AU - Ueno, Takahiro

AU - Soma, Masayoshi

AU - Nagase, Hiroki

PY - 2014

Y1 - 2014

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AB - Gene amplification and/or overexpression of the transcription factor c-MYC, which binds to the E-box sequence (5'-CACGTG-3'), has been observed in many human tumors. In this study, we have designed 5 pyrrole-imidazole (PI) polyamides recognizing E-box, and found that, among them, Myc-6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc-6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc-6 exerts its tumor-suppressive ability at least in part through the specific down-regulation of MALAT1.

KW - E-box

KW - MALAT1

KW - MG63 cells

KW - Osteosarcoma

KW - Pyrrole-imidazole polyamide

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JO - FEBS Open Bio

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ER -

Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole-imidazole polyamide, which targets an E-box motif

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