Imaging Analysis Enables Differentiation of the Distribution of Pharmaceutical Ingredients in Tacrolimus Ointments

Mika Yoshimura Fujii, Yoshihisa Yamamoto, Tatsuo Koide, Masashi Hamaguchi, Yoshinori Onuki, Naoto Suzuki, Toyofumi Suzuki, Toshiro Fukami

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We demonstrated the difference in the distribution state of pharmaceutical ingredients between tacrolimus (TCR) original ointment and six kinds of generic medicines. Two-dimensional imaging and depth analysis using attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy and confocal Raman microscopy were used, in addition to the evaluation of pharmaceutical properties, including spreading properties, rheological properties, and amount of solvent. The solvents, such as propylene carbonate and triacetin, in TCR ointments formed liquid droplets and dispersed in hydrocarbon oils. Waxes, white beeswax and beeswax, formed other domains. Confocal Raman microscopy could detect liquid droplet size without coalescence of that on germanium or glass surfaces. The combination of ATR FT-IR and confocal Raman imaging would be a powerful tool to reveal the size and shape of liquid droplets of pharmaceutical ingredients in semisolid formulations.

Original languageEnglish
Pages (from-to)1183-1192
Number of pages10
JournalApplied Spectroscopy
Volume73
Issue number10
DOIs
Publication statusPublished - 1 Oct 2019

Keywords

  • confocal Raman spectroscopy
  • imaging analysis
  • rheological property
  • Tacrolimus ointments

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