IgG-mediated histamine release from canine mastocytoma-derived cells

Tomoko Takahashi, Seiichi Kitani, Masayuki Nagase, Manabu Mochizuki, Ryohei Nishimura, Yutaka Morita, Nobuo Sasaki

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Background: Recent data suggest that normal tissue mast cells can express functional receptors for IgG under certain conditions. However, little is known about IgG receptor expression and functional consequences in mast cell neoplasms. Methods: In this study, neoplastic mast cells were obtained from a dog with cutaneous mastocytoma (CM-MC) and from a dog with visceral mastocytoma (VI-MC). Both cell populations were characterized morphologically and functionally. Results: Most cells proliferated constantly in suspension without particular supplements. Doubling times of CM-MC and VI-MC were 52.2 and 27.5 h, respectively. Both cell types were sensitive to formalin fixation, did not contain heparin and were tryptase and chymase positive. Electron microscopy showed fine granules with electron-dense content in both cell populations. The total histamine content of CM-MC and VI-MC was 0.25 and 0.10 pg/cell, respectively. Calcium ionophore A23187 and substance P induced dose-dependent histamine release, whereas compound 48/80 had no effect. Most significantly, both cell types, when sensitized with monomeric dog IgG, released histamine upon stimulation by anti-dog IgG. Conclusions: Dog mastocytoma-derived cells may be useful to study the regulation of neoplastic mast cell growth and differentiation, as well as IgG receptor-mediated activation in neoplastic mast cells. Further research is required to clarify the pathophysiological significance of constitutive expression of IgG receptors in neoplastic (canine) mast cells.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume125
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Canine mastocytoma
  • Chymase
  • Histamine release
  • IgG receptor
  • Tryptase

Fingerprint

Dive into the research topics of 'IgG-mediated histamine release from canine mastocytoma-derived cells'. Together they form a unique fingerprint.

Cite this