Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma

Kentaro Sakuma; Atsushi Kasamatsu; Masanobu Yamatoji; Yukio Yamano; Kazuaki Fushimi; Manabu Iyoda; Kenji Ogoshi; Keiji Shinozuka; Katsunori Ogawara; Masashi Shiiba; Hideki Tanzawa; Katsuhiro Uzawa

doi:10.1007/s00432-009-0689-y

Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma

Kentaro Sakuma
, Atsushi Kasamatsu
, Masanobu Yamatoji
, Yukio Yamano
, Kazuaki Fushimi
, Manabu Iyoda
, Kenji Ogoshi
, Keiji Shinozuka
, Katsunori Ogawara
, Masashi Shiiba
, Hideki Tanzawa
, Katsuhiro Uzawa

Chiba University

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.

Original language	English
Pages (from-to)	553-559
Number of pages	7
Journal	Journal of Cancer Research and Clinical Oncology
Volume	136
Issue number	4
DOIs	https://doi.org/10.1007/s00432-009-0689-y
Publication status	Published - Apr 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Immunohistochemistry
Oral premalignant lesion
Oral squamous cell carcinoma
QRT-PCR
Zic family member 2

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10.1007/s00432-009-0689-y

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title = "Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma",

abstract = "Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.",

keywords = "Immunohistochemistry, Oral premalignant lesion, Oral squamous cell carcinoma, QRT-PCR, Zic family member 2",

author = "Kentaro Sakuma and Atsushi Kasamatsu and Masanobu Yamatoji and Yukio Yamano and Kazuaki Fushimi and Manabu Iyoda and Kenji Ogoshi and Keiji Shinozuka and Katsunori Ogawara and Masashi Shiiba and Hideki Tanzawa and Katsuhiro Uzawa",

year = "2010",

month = apr,

doi = "10.1007/s00432-009-0689-y",

language = "English",

volume = "136",

pages = "553--559",

journal = "Journal of Cancer Research and Clinical Oncology",

issn = "0171-5216",

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TY - JOUR

T1 - Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma

AU - Sakuma, Kentaro

AU - Kasamatsu, Atsushi

AU - Yamatoji, Masanobu

AU - Yamano, Yukio

AU - Fushimi, Kazuaki

AU - Iyoda, Manabu

AU - Ogoshi, Kenji

AU - Shinozuka, Keiji

AU - Ogawara, Katsunori

AU - Shiiba, Masashi

AU - Tanzawa, Hideki

AU - Uzawa, Katsuhiro

PY - 2010/4

Y1 - 2010/4

N2 - Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.

AB - Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.

KW - Immunohistochemistry

KW - Oral premalignant lesion

KW - Oral squamous cell carcinoma

KW - QRT-PCR

KW - Zic family member 2

UR - https://www.scopus.com/pages/publications/77949273886

U2 - 10.1007/s00432-009-0689-y

DO - 10.1007/s00432-009-0689-y

M3 - Article

C2 - 19784848

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SN - 0171-5216

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EP - 559

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

IS - 4

ER -

Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma

Abstract

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Keywords

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