TY - JOUR
T1 - Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma
AU - Sakuma, Kentaro
AU - Kasamatsu, Atsushi
AU - Yamatoji, Masanobu
AU - Yamano, Yukio
AU - Fushimi, Kazuaki
AU - Iyoda, Manabu
AU - Ogoshi, Kenji
AU - Shinozuka, Keiji
AU - Ogawara, Katsunori
AU - Shiiba, Masashi
AU - Tanzawa, Hideki
AU - Uzawa, Katsuhiro
PY - 2010/4
Y1 - 2010/4
N2 - Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.
AB - Purpose: To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC). Methods: ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status. Results: Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases. Conclusions: These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.
KW - Immunohistochemistry
KW - Oral premalignant lesion
KW - Oral squamous cell carcinoma
KW - QRT-PCR
KW - Zic family member 2
UR - http://www.scopus.com/inward/record.url?scp=77949273886&partnerID=8YFLogxK
U2 - 10.1007/s00432-009-0689-y
DO - 10.1007/s00432-009-0689-y
M3 - Article
C2 - 19784848
AN - SCOPUS:77949273886
SN - 0171-5216
VL - 136
SP - 553
EP - 559
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 4
ER -
