Expression and localization of laminin 5, laminin 10, type IV collagen, and amelotin in adult murine gingiva

Takashi Sawada, Takaki Yamazaki, Kazuko Shibayama, Kaido Kumazawa, Yoko Yamaguchi, Mitsuhiro Ohshima

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The biochemical composition of the internal and external basal laminae in the junctional epithelium differs significantly, and the precise cellular origin of their respective molecules remains to be determined. In the present study, the expression and localization of three basement membrane-specific molecules-laminin 5 (γ2 chain), type IV collagen (α1 chain), and laminin 10 (α5 chain)-and one tooth-specific molecule, amelotin, was analyzed in adult murine gingiva by using in situ hybridization and immunohistochemistry. The results showed that the outermost cells in junctional epithelium facing the tooth enamel strongly expressed laminin 5 mRNA, supporting the immunohistochemical staining data. This suggests that laminin 5 is actively synthesized in junctional epithelial cells and that the products are incorporated into the internal basal lamina to maintain firm epithelial adhesion to the tooth enamel throughout life. Conversely, no amelotin mRNA signals were detected in the junctional epithelial cells, suggesting that the molecules localized on the internal basal lamina are mainly derived from maturation-stage ameloblasts. Weak and sporadic expression of type IV collagen in addition to laminin 10 in the gingiva indicates that these molecules undergo turnover less frequently in adult animals.

Original languageEnglish
Pages (from-to)293-302
Number of pages10
JournalJournal of Molecular Histology
Volume45
Issue number3
DOIs
Publication statusPublished - Jun 2014

Keywords

  • Amelotin
  • Gingiva
  • Immunohistochemistry
  • In situ hybridization
  • Laminins
  • Type IV collagen

Fingerprint

Dive into the research topics of 'Expression and localization of laminin 5, laminin 10, type IV collagen, and amelotin in adult murine gingiva'. Together they form a unique fingerprint.

Cite this