Effect of high-concentrate corn grain diet-induced elevated ruminal lipopolysaccharide levels on dairy cow liver function

Tadatoshi Ohtaki, Kanae Ogata, Hiroshi Kajikawa, Toshiaki Sumiyoshi, Sanae Asano, Shigehisa Tsumagari, Tetsuya Horikita

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


A high-concentrate diet destroys gram-negative bacteria in the cattle rumen, leading to elevated ruminal lipopolysaccharide (LPS) levels. LPS causes liver inflammation through the hepatic portal vein but little is known about the effects of rumen-derived LPS on liver function and the reproductive organs. In this study, we determined the effect of increasing rumen fluid LPS levels on liver function and genital LPS levels. Cows were assigned to control (CON; n=5) and high-concentrate diet (HC; n=7) groups. We observed that the ruminal LPS and haptoglobin (Hp) levels were significantly higher and albumin levels were lower in the HC group than in the CON group. In the HC group, The Hp levels and aspartate transaminase (AST) activity were significantly higher and the total cholesterol levels were significantly lower after high-concentrate diet feeding than before feeding. No differences were observed in LPS levels in the peripheral veins, hepatic veins, hepatic portal vein, uterine perfusate, and follicular fluids between the groups. In all samples, the LPS level in the hepatic portal vein blood positively correlated with the AST activity and serum amyloid A level. In conclusion, our results indicate that high-concentrate diets do not have a direct effect on the reproductive organs upon a moderate ruminal LPS level increase. However, an increased ruminal LPS influx into the liver might affect negatively liver function.

Original languageEnglish
Pages (from-to)971-977
Number of pages7
JournalJournal of Veterinary Medical Science
Issue number7
Publication statusPublished - Jul 2020


  • High-concentrate diet
  • Lipopolysaccharide
  • Liver
  • Reproductive organ
  • Rumen


Dive into the research topics of 'Effect of high-concentrate corn grain diet-induced elevated ruminal lipopolysaccharide levels on dairy cow liver function'. Together they form a unique fingerprint.

Cite this