DNA typing of the D1S80 locus using capillary zone electrophoresis

Jian Tie; Shojiro Tsukamoto; Shigemi Oshida; Xiuling Wang; Ismail M. Sebetan; Shoetsu Chiba; Masao Shimamura

doi:10.1016/S0379-0738(97)00048-0

DNA typing of the D1S80 locus using capillary zone electrophoresis

Jian Tie, Shojiro Tsukamoto, Shigemi Oshida, Xiuling Wang, Ismail M. Sebetan, Shoetsu Chiba, Masao Shimamura

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.

Original language	English
Pages (from-to)	193-199
Number of pages	7
Journal	Forensic Science International
Volume	87
Issue number	3
DOIs	https://doi.org/10.1016/S0379-0738(97)00048-0
Publication status	Published - 23 Jun 1997

Keywords

CZE
D1S80 locus
Typing

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10.1016/S0379-0738(97)00048-0

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title = "DNA typing of the D1S80 locus using capillary zone electrophoresis",

abstract = "DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.",

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T1 - DNA typing of the D1S80 locus using capillary zone electrophoresis

AU - Tie, Jian

AU - Tsukamoto, Shojiro

AU - Oshida, Shigemi

AU - Wang, Xiuling

AU - Sebetan, Ismail M.

AU - Chiba, Shoetsu

AU - Shimamura, Masao

PY - 1997/6/23

Y1 - 1997/6/23

N2 - DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.

AB - DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.

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KW - D1S80 locus

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DNA typing of the D1S80 locus using capillary zone electrophoresis

Abstract

Keywords

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