TY - JOUR
T1 - Distribution of aquaporin-5, transforming growth factor-β1 and laminin during regeneration of atrophic rat submandibular glands after duct ligation
AU - Yasumitsu, Tomohiro
AU - Shimizu, Osamu
AU - Shiratsuchi, Hiroshi
AU - Miyake, Yusuke
AU - Yonehara, Yoshiyuki
N1 - Publisher Copyright:
© 2018, Nihon University, School of Dentistry. All rights reserved.
PY - 2018/12
Y1 - 2018/12
N2 - The aim of this study was to determine the localization of aquaporin-5 (AQP5), transforming growth factor-β1 (TGF-β1) and laminin during regeneration of the rat submandibular gland. After duct ligation for 7 days, the regenerating glands were collected on days 0, 1, 3, 7, and 14 after ligation release to study the process of regeneration. Immunohistochemical staining revealed apical expression of AQP5 in many acinar cells, strong expression in intercalated ducts (ICDs) of the normal submandibular gland at Day 14, and strong expression in duct-like structures (DLSs) during regeneration from Day 0 to 7. However, a few AQP5-negative acinar cells were detected during regeneration. At Day 0, immu-nopositivity for TGF-β1 was detected in connective tissue. At Days 3 and 7 during regeneration, TGF-β1 immunostaining was observed in DLSs, which were surrounded by α-smooth muscle actin-positive thickened myoepithelial cells. Laminin staining was strong in the thickened basement membrane of DLSs at Day 3 during regeneration, but weak around acinar cells at Day 14. These findings suggest that TGF-β1 is involved in the environment around DLSs, myoepithelial cells and laminin, that DLSs have the same functional properties as ICDs, and that AQP5-negative acinar cells may be mucous cells.
AB - The aim of this study was to determine the localization of aquaporin-5 (AQP5), transforming growth factor-β1 (TGF-β1) and laminin during regeneration of the rat submandibular gland. After duct ligation for 7 days, the regenerating glands were collected on days 0, 1, 3, 7, and 14 after ligation release to study the process of regeneration. Immunohistochemical staining revealed apical expression of AQP5 in many acinar cells, strong expression in intercalated ducts (ICDs) of the normal submandibular gland at Day 14, and strong expression in duct-like structures (DLSs) during regeneration from Day 0 to 7. However, a few AQP5-negative acinar cells were detected during regeneration. At Day 0, immu-nopositivity for TGF-β1 was detected in connective tissue. At Days 3 and 7 during regeneration, TGF-β1 immunostaining was observed in DLSs, which were surrounded by α-smooth muscle actin-positive thickened myoepithelial cells. Laminin staining was strong in the thickened basement membrane of DLSs at Day 3 during regeneration, but weak around acinar cells at Day 14. These findings suggest that TGF-β1 is involved in the environment around DLSs, myoepithelial cells and laminin, that DLSs have the same functional properties as ICDs, and that AQP5-negative acinar cells may be mucous cells.
KW - Aquaporin-5
KW - Duct-like structures
KW - Laminin
KW - Rat submandibular gland
KW - Regeneration
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=85059238415&partnerID=8YFLogxK
U2 - 10.2334/josnusd.17-0491
DO - 10.2334/josnusd.17-0491
M3 - Article
C2 - 30587691
AN - SCOPUS:85059238415
SN - 1343-4934
VL - 60
SP - 595
EP - 600
JO - Journal of Oral Science
JF - Journal of Oral Science
IS - 4
ER -