Distinct roles of Lactobacillus species in trophoblast invasion: Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus

Jonghyun Bae; Kazuhide Takada; Yoshinori Takeda; Quang Duy Trinh; Shihoko Komine-Aizawa; Dong Wook Kim; Satoshi Hayakawa

doi:10.1016/j.repbio.2025.101041

Distinct roles of Lactobacillus species in trophoblast invasion: Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus

Jonghyun Bae
, Kazuhide Takada
, Yoshinori Takeda
, Quang Duy Trinh
, Shihoko Komine-Aizawa
, Dong Wook Kim
, Satoshi Hayakawa

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The role of Lactobacillus iners in the uterus during pregnancy is poorly understood. This study aimed to evaluate the effect of L. iners on trophoblast invasion. The HTR-8/SVneo cell line was cultured with culture supernatants from L. iners (SLI) or L. crispatus (SLC). SLC significantly enhanced HTR-8/SVneo invasion, which was accompanied by increased matrix metalloproteinase-2 mRNA expression, protein expression, and enzymatic activity. In contrast, SLI did not affect trophoblast invasion. These results suggest that the presence of L. iners in the endometrium is less favorable than that of L. crispatus for promoting trophoblast invasion during early pregnancy.

Original language	English
Article number	101041
Journal	Reproductive Biology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/j.repbio.2025.101041
Publication status	Published - Sept 2025

Keywords

Endometrial microbiota
Implantation
Lactobacillus
Trophoblast invasion

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10.1016/j.repbio.2025.101041

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title = "Distinct roles of Lactobacillus species in trophoblast invasion: Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus",

abstract = "The role of Lactobacillus iners in the uterus during pregnancy is poorly understood. This study aimed to evaluate the effect of L. iners on trophoblast invasion. The HTR-8/SVneo cell line was cultured with culture supernatants from L. iners (SLI) or L. crispatus (SLC). SLC significantly enhanced HTR-8/SVneo invasion, which was accompanied by increased matrix metalloproteinase-2 mRNA expression, protein expression, and enzymatic activity. In contrast, SLI did not affect trophoblast invasion. These results suggest that the presence of L. iners in the endometrium is less favorable than that of L. crispatus for promoting trophoblast invasion during early pregnancy.",

keywords = "Endometrial microbiota, Implantation, Lactobacillus, Trophoblast invasion",

author = "Jonghyun Bae and Kazuhide Takada and Yoshinori Takeda and Trinh, \{Quang Duy\} and Shihoko Komine-Aizawa and Kim, \{Dong Wook\} and Satoshi Hayakawa",

note = "Publisher Copyright: {\textcopyright} 2025 Society for Biology of Reproduction \& the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn",

year = "2025",

month = sep,

doi = "10.1016/j.repbio.2025.101041",

language = "English",

volume = "25",

journal = "Reproductive Biology",

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T1 - Distinct roles of Lactobacillus species in trophoblast invasion

T2 - Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus

AU - Bae, Jonghyun

AU - Takada, Kazuhide

AU - Takeda, Yoshinori

AU - Trinh, Quang Duy

AU - Komine-Aizawa, Shihoko

AU - Kim, Dong Wook

AU - Hayakawa, Satoshi

PY - 2025/9

Y1 - 2025/9

N2 - The role of Lactobacillus iners in the uterus during pregnancy is poorly understood. This study aimed to evaluate the effect of L. iners on trophoblast invasion. The HTR-8/SVneo cell line was cultured with culture supernatants from L. iners (SLI) or L. crispatus (SLC). SLC significantly enhanced HTR-8/SVneo invasion, which was accompanied by increased matrix metalloproteinase-2 mRNA expression, protein expression, and enzymatic activity. In contrast, SLI did not affect trophoblast invasion. These results suggest that the presence of L. iners in the endometrium is less favorable than that of L. crispatus for promoting trophoblast invasion during early pregnancy.

AB - The role of Lactobacillus iners in the uterus during pregnancy is poorly understood. This study aimed to evaluate the effect of L. iners on trophoblast invasion. The HTR-8/SVneo cell line was cultured with culture supernatants from L. iners (SLI) or L. crispatus (SLC). SLC significantly enhanced HTR-8/SVneo invasion, which was accompanied by increased matrix metalloproteinase-2 mRNA expression, protein expression, and enzymatic activity. In contrast, SLI did not affect trophoblast invasion. These results suggest that the presence of L. iners in the endometrium is less favorable than that of L. crispatus for promoting trophoblast invasion during early pregnancy.

KW - Endometrial microbiota

KW - Implantation

KW - Lactobacillus

KW - Trophoblast invasion

UR - https://www.scopus.com/pages/publications/105006948632

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DO - 10.1016/j.repbio.2025.101041

M3 - Article

C2 - 40460761

AN - SCOPUS:105006948632

SN - 1642-431X

VL - 25

JO - Reproductive Biology

JF - Reproductive Biology

IS - 3

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ER -

Distinct roles of Lactobacillus species in trophoblast invasion: Lactobacillus iners fails to promote HTR-8/SVneo cell invasion unlike Lactobacillus crispatus

Abstract

Keywords

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